email: mgef [at] ncbs [dot] res [dot] in

2) MOUSE GENOME ENGINEERING FACILITY services:

Currently available services:

- Help in strategic experimental design for mouse Transgenesis / Knock-In / Knock-Out (KO & cKO).
- Pronuclear and/or cytoplasmic microinjections in fertilized mouse oocytes.
- The facility can inject various types of constructs including: BACs, plasmids, CRISPR/cas, TALENs...
- Harvesting of mouse oocytes , blastocysts, other preimplantation embryos
- Embryo transfer into pseudo-pregnant females & rederivation of mouse lines.
The facility offers both Infundibulum/oviduct and uterine embryo transfer depending on project specifics
- Final gel purification of the “micro-injection solutions”

- Sperm  cryopreservation / cryo-recovery
- IVF (In Vitro Fertilization)

- Embryo cryopreservation / cryo-recovery

- Training and workshops on all the above mentioned techniques....please visit our <workshop page>

molecular biology / genome engineering construct design and validation

- genotyping

 

Future Planned MGEF services:

- mES cell gene editing and microinjections

- genetic monitoring

- ICSI (intracytoplasmic sperm injection)

- ovary transplantation

- Rat trangenesis and Rat IVF