Electron Microscopy Facility - TEM Techniques
1. Bright field Image: The bright field imaging mode is the most common mode of operation for TEM where the image is formed by the direct interaction of the beam with the specimen. Mass-thickness and diffraction contrast contribute to image formation in this mode and thus thick areas in which heavy atoms are enriched, and crystalline areas appear dark.
2.Cryo TEM: Cryo TEM imaging is a technique that can be applied to unique objects such as flexible large protein complexes, irregular viruses,organells and small cells for imaging at cryogenic temperatures. Specimens are preserved in a near native, 'frozen-hydrated' state by vitrification process using a dedicated plunge freezing setup.
3. Tomography: Tomograghy technique is used to obtain detailed 3D structures of sub-cellular macro-molecular objects, on the basis of multiple 2D projection images of a 3D objects, obtained over a wide range of veiwing directions (+75° to -75°)