Since 2009 the Fly Facility, has provided services to facilitate modern molecular genetics in Drosophila. Please use the form at https://www.ccamp.res.in/FlyFacility to avail the services.

1. PhiC31integrase mediated transgenesis:

 

Site-Specific Excel - Injection of user generated DNA into upto 300 embryos of flies containing PhiC31 integrase & selected attP docking site (click here and choose attP sites), crossing of G0 flies, screening the transformants and balancing the insertions.

 

Site-Specific BAC - This service includes injecting the BACs or fosmids (>30kb) into 500 embryos of strain containing PhiC31-integrase and an AttP docking site of the user’s choice and scoring the transformants, balancing and shipping.

 

Site-Specific CRISPR - This service includes injecting the plasmids with CRISPR/Cas9 guide RNA plasmid into upto 300 embryos of strain containing PhiC31-integrase and a docking site of the user’s choice and scoring the transformants, balancing and shipping.

 

2. CRISPR/Cas9 mediated mutagenesis:

 

gRNA design and synthesis - This service includes design of a single gRNA and its synthesis in a plasmid vector available in the facility (pBFVU6.2 or pCFD). User will get the plasmid DNA and the sequencing data.

 

Testing gRNA pairs in embryos - This service include injection of gRNA pairs in 50 embryos of Act5c-Cas9 transgenic, and PCR based screening for the efficiency of the pair.

 

Large (2kb+ deletions) - This service includes injecting dual-gRNA in ~300 embryos from appropriate Cas9 flies, G0 crosses, PCR based screening, sequence confirmation and balancing mutant flies.

 

Homologous recombination construct design and synthesis - This service includes design and synthesis of HR construct by Gibson Assembly. User will get the final construct and the sequencing data.

 

CRISPR based homologous recombination - This service includes injection of gRNA and HR construct provided by the user, crossing G0 flies and screening for visible markers and balancing the positive progeny.

 

3. Embryo Microinjection service:

 

This rapid mode service includes injection of user supplied DNA in 300 embyos in Standard Drosophila stocks.

 

*Injection in Non-standard stocks for any of the services may be requested. This additional service will require stock expansion and will be charged extra.

 

4. P-element mediated transgensis:

 

P-element classic - Injection of DNA into w1118 embryos, raising the survived larvae to adulthood and crossing the G0 flies (>100 individual crosses). Identification and collection of transformants; the collected transformant flies (at least 3 independent lines) are sent to the customer and original vials are maintained in the facility for two weeks as backup.

 

P-element excel - The service includes mapping of the transformants obtained in addition to service in P-element classic.

 

 

5. Custom services are also available on request

 

Please write to us for further details.