Research Overview

A number of epigenetic regulatory layers are superimposed on the genome. In plants, small RNA regulators play a major role in the establishment and maintenance of epigenetic marks. We are interested in understanding the mechanism of small RNA biogenesis, their functions and their role in establishment of epigenetics using a number of model systems.

Link to our lab webpage

Dr  P V  Shivaprasad

Group Leader

National Centre for Biological Sciences

Tata Institute of Fundamental Research (TIFR), India

E-mail: shivaprasad at ncbs dot res dot in


Office: +91-80-2366-6511


M.Sc. Applied Botany, Mangalore University

PhD, Biotechnology, Madurai Kamaraj University

Postdoctoral Research (EMBO and SNF), Viral suppressors of gene silencing, Prof. Thomas Hohn's lab, University of Basel

Postdoctoral Research (BBSRC grant), Gene silencing in tomato, Prof. Sir. David Baulcombe's lab, University of Cambridge

Expertise Summary

Molecular analysis of plants:- Next Gen Sequencing, forward and reverse genetics, transcriptomics, ChIP analysis, whole-genome methylation analysis, biotechnology, use of multiple model plants to study functions of small RNAs and epigenetics using molecular, genetic, bioinformatic and biochemical approaches.

Teaching Summary

Basic Biology-plants, RNA biology, Plant development, Disease biology and Genetics.

Selected Publications

Major domestication-related phenotypes in indica rice are due to loss of miRNA-mediated laccase silencing Chenna Swetha, Debjani Basu, Kannan Pachamuthu, Varsha Tirumalai, Ashwin Nair, Melvin Prasad, Padubidri V. Shivaprasad - The Plant Cell, 2018 (Breakthrough Report)  [Press Release]

Molecular characterization of a new begomovirus infecting Synedrella nodiflora in South India S Das, A Hegde, PV Shivaprasad - Archives of virology, 2018 

Methylglyoxal detoxification by a DJ-1 family protein provides dual abiotic and biotic stress tolerance in transgenic plants P Melvin, K Bankapalli, P D’Silva, PV Shivaprasad Plant Molecular Biology (2017)

Biochemical requirements for two dicer-like activities from wheat germ PV Shivaprasad, T Hohn, R Akbergenov PloS One (2015).

Diversity, expression and mRNA targeting abilities of Argonaute-targeting miRNAs among selected vascular plants S Jagtap, PV Shivaprasad BMC Genomics (2014)

A microRNA superfamily regulates nucleotide binding site–leucine-rich repeats and other mRNAs PV Shivaprasad et al, The Plant Cell (2012).

Extraordinary transgressive phenotypes of hybrid tomato are influenced by epigenetics and small silencing RNAs PV Shivaprasad et al, The EMBO journal (2012).

The RNAi targeting of DNA virus in plants Poggin, Mikhail, P.V. Shivaprasad, K. Veluthambi, and Thomas Hohn - Nature Biotechnology (2003).

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