Motor Function of the Two-Component EEA1-Rab5 Revealed by dcFCCS.
| Title | Motor Function of the Two-Component EEA1-Rab5 Revealed by dcFCCS. |
| Publication Type | Journal Article |
| Year of Publication | 2025 |
| Authors | Soler JAntoni, Singh A, Zerial M, Thutupalli S |
| Journal | Methods Mol Biol |
| Volume | 2881 |
| Pagination | 87-115 |
| Date Published | 2025 |
| ISSN | 1940-6029 |
| Keywords | Humans, rab5 GTP-Binding Proteins, Single Molecule Imaging, Spectrometry, Fluorescence, Vesicular Transport Proteins |
| Abstract | Fluorescence correlation spectroscopy (FCS) enables the measurement of fluctuations at fast timescales (typically few nanoseconds) and with high spatial resolution (tens of nanometers). This single-molecule measurement has been used to characterize single-molecule transport and flexibility of polymers and biomolecules such as DNA and RNA. Here, we apply this technique as dual-color fluorescence cross-correlation spectroscopy (dcFCCS) to identify the motor function of the tethering protein EEA1 and the small GTPase Rab5 by probing the flexibility changes through end-monomer fluctuations. |
| DOI | 10.1007/978-1-0716-4280-1_4 |
| Alternate Journal | Methods Mol Biol |
| PubMed ID | 39704939 |
| PubMed Central ID | 26734 |