NCBS-inStem-IC post-doc partnership program

The National Centre for Biological Sciences (NCBS), Bangalore, the Institute for Stem Cell Biology and Regenerative Medicine (inStem), Bangalore, and Institut Curie, France (IC) are pleased to announce the NCBS/inStem/IC Postdoctoral Fellowship (NCBS/inStem/IC-PDF).

The program will provide excellent training to scientists through a structured exchange program to encourage scientific collaborations between IC , Paris, France and NCBS/inStem , Bangalore, India.

Each year there will be a call for collaborative projects, which must be proposed by two PIs, whereby one PI is located at the IC, and the other at the NCBS/inStem.

Two year joint post doctoral fellowship with annual review and renewal. Under exceptional circumstances the duration can be extended for one-year durations up to a maximum of four years.

The Basic salary for the joint postdoctoral fellow will be paid by NCBS/inStem, commensurate with the terms of the Campus Fellows Program and subject to tax. Institut Curie will provide top-up salary to the fellow to cover his/her costs of living for the duration of their stay in Paris for the project. Fellows should spend approximately equal amounts of time in Bangalore and Paris.

 

Interested candidates should directly apply to the participating PIs of the joint projects listed here.

The application package should consist of the following:

  • Your latest CV

  • PDF versions of 1-3 first-author publications.

  • At least 2 letters of references (e-mailed directly to the participating PIs)

If shortlisted by the participating PIs, you should develop a research proposal with detailed plans and timelines of the project for 4 years, developed in close collaboration with your proposed hosts.

Full applications consisting of the following should be sent by e-mail as a single pdf package to acadoffice@ncbs.res.in.

  • Your latest CV

  • A research proposal with detailed plans and timelines developed for one of the Joint Project Blurbs. The length should not be more than 4-5 pages including references.

  • PDF versions of 1-3 first-author publications.

  • At least 2 letters of references/recommendations (e-mailed directly to acadoffice@ncbs.res.in)

  • Host PIs support letter (e-mailed directly to acadoffice@ncbs.res.in)

Deadline for sending the complete application to acadoffice@ncbs.res.in : 31st March 2019.

Decisions about the short-listed candidate will be announced within 2 weeks after this deadline.

JOINT PROJECT NO. 1

Mechanistic Understanding of Clathrin and Dynamin-Independent Endocytic Pathways

PIs: Satyajit Mayor, NCBS, Bangalore, India ( mayor@ncbs.res.in ) and Ludger Johannes, Institut Curie, Paris, France (ludger.johannes@curie.fr)

It is evident that several endocytic mechanisms exist for the internalization of extracellular or plasma membrane proteins, and that are necessary for nutrient uptake, cell signaling and infection. While the importance of clathrin and dynamin-dependent mechanisms is well-established, the roles of clathrin and dynamin-independent mechanisms is only now being recognised. The laboratories of Satyajit Mayor at the National Centre for Biological Science (TIFR) (NCBS-TIFR, Bangalore, India) and Ludger Johannes at Institut Curie (IC, Paris, France) have uncovered new molecular machinery for these processes . While the Mayor laboratory has focused on the uptake of GPI-anchored proteins via the CLIC/GEEC pathway, and the Johannes laboratory on bacterial and cellular lectins such as Galectin-3 and their interactions with glycosphingolipids and glycosylated cargo proteins via the GlycoLipid-Lectin (GL-Lect) mechanism. In the current joint program between NCBS and IC, we wish to use state-of-the art live cell imaging (such as lattice light sheet microscopy at IC, TIRF combined with pH pulsing and fluorescence emission anisotropy imaging at NCBS) and advanced cellular biochemistry techniques (such as DNA nanotechnology-based endocytic carrier purification) for the molecular dissection of the mechanisms that lead to the clathrin-independent formation of endocytic pits responsible for the uptake of these cargo. We expect to uncover general principles that would be common to mechanisms for building endocytic pits without involvement of the clathrin and dynamin machinery. The mechanism of uptake of these cargo will also provide insights into the clathrin-independent membrane bending and dynamin-independent endocytic scission processes.

The NCBS-IC post-doctoral fellow will formulate a project in close collaboration with the two laboratories, and work at both sites during the duration of the fellowship, depending on the demands of the science.

Please send your application (CV, a list of publications and two reference letters) by email to mayor@ncbs.res.in, ludger.johannes@curie.fr, before 15th February 2019.

Key publications:

Thottacherry, J. J. et al. (2018) Mechanochemical feedback control of dynamin independent endocytosis modulates membrane tension in adherent cells. Nat. Commun. 9, 4217.

Sathe, M. et al. (2018) Small GTPases and BAR domain proteins regulate branched actin polymerisation for clathrin and dynamin-independent endocytosis. Nat. Commun. 9, 1835.

Pezeshkian et al. (2017) Mechanism of Shiga toxin clustering on membranes. ACS Nano 11: 314-324

Shafaq-Zadah M, et.al. (2016) Persistent cell migration and adhesion rely on retrograde transport of beta1 integrin. Nat Cell Biol 18: 54-64

Renard H-F, et. al. (2015) Endophilin-A2 functions in membrane scission in clathrin-independent endocytosis. Nature 517: 493-496

Johannes L, Parton RG, Bassereau P, Mayor S (2015) Building endocytic pits without clathrin. Nat Rev Mol Cell Biol 16: 311-321

Lakshminarayan R. et.al (2014) Galectin-3 drives glycosphingolipid-dependent biogenesis of clathrin-independent carriers. Nat Cell Biol 16: 595-606

 

JOINT PROJECT NO. 2

Determining the mechanism of tubulin glycylation in stabilizing cilia and flagella

PIs: Minhaj Sirajuddin, inStem, Bangalore, India (minhaj@instem.res.in) and Carsten Janke, Institut Curie, Paris, France (carsten.janke@curie.fr)

Microtubules are ubiquitous cytoskeletal elements with a variety of functions, the basic subunit, tubulin undergoes various posttranslational modifications (PTMs), which are involved in the determination of microtubule properties and functions. Among all known PTMs, glycylation is unique as it occurs only on axonemes, a highly specialized microtubule structures that build cilia and flagella. The Janke and Sirajuddin labs have previously demonstrated the importance of some tubulin PTMs in regulating motor activity, MAP binding and severing enzymes, however no molecular mechanisms has been identified for glycylation. So far we have shown that in vivo, glycylation is involved in the stabilization of cilia. To understand how glycylation can achieve this stabilization, we will now develop in vitro studies, which we will combine with cellular and in vivo approaches, to directly determine the molecular mechanism(s) by which glycylation stabilizes the microtubule axoneme in cilia and flagella. The in vitro approach will involve purifying tubulin/microtubules with defined glycylation patterns, as well as measuring the intraflagellar anterograde and retrograde motor activity, the stability of microtubules, and severing assays. Our two labs have established the complementary methodology to successfully perform the collaborative project. The Janke lab has established cell and mouse models for glycylation, and installed a pipeline for the production of à-la-carte tubulin for in vitro assays. The Sirajuddin team has a strong expertise in in vitro assays (motors, MAPs, severing enzymes), as well as in structural biology. The proposed project will complement our efforts in tackling the still enigmatic role of glycylation.

Please send your application (CV, a list of publications and two reference letters) by email to minhaj@instem.res.in, carsten.janke@curie.fr, before 15th February 2019.

 

JOINT PROJECT NO. 3

We seek highly motivated, outstanding and adventurous candidates from the engineering and physical sciences for two (theoretical and experimental) NCBS-inStem-IC Postdoctoral Fellowships to investigate challenging problems in the Physics of Living Systems.

We propose to use the principles of soft condensed matter physics, fluid mechanics, and non-equilibrium physics to study the structure and form of complex compartments within the cell. Specifically, we are interested in the control of size, shape and spatial positioning of cellular organelles within the trafficking pathway, which are subject to active fission and fusion events. We view such cellular compartments as steady states of a driven non-equilibrium process, which we will investigate theoretically and experimentally.

Theory: We have recently constructed the covariant hydrodynamics of a closed membrane embedded in a Stokesian fluid which is subject to active fission and fusion events. These equations, solved analytically in simple settings, already reveal several exciting results, such as a generic drift instability of the organelle. To explore the full scope of these hydrodynamic equations, we would like to use numerical schemes, such as Boundary Element Methods (BEM) or Finite Element Methods (FEM). We are looking for adventurous and talented post-doctoral fellows who are familiar with or willing to learn these methods.

Experiment: We aim to build in vitro model lipid membrane systems incorporating membrane fusion, sorting and spontaneous scission using a combination of microfluidics and soft matter techniques. Using these basic ingredients, we will study the de novo emergence of features of complex organelles. Of particular emphasis will be the quantitative control of the transport, fusion, fission and segregation of the structures commensurate with in vitro studies. These experiments will be carried out in close collaboration with the theoretical investigations above. We are looking for expertise in artificial membrane systems, microfluidics, microscopy, optical manipulation and related physical techniques. Persons with a theoretical background with a strong interest in building experiments are also encouraged to apply.

Our offer

This is a joint research program between theorists (Prof. Madan Rao and Prof. Pierre Sens) and experimentalists (Prof. Patricia Bassereau and Dr. Shashi Thutupalli) at two institutes (National Centre for Biological Sciences, Bangalore, India and the Institut Curie, Paris, France).

Right from the start, the candidate will be encouraged and even expected to develop a great degree of independence — from drafting the initial details of the project within the broad framework outlined and to propose interesting directions going forward.

Your application

Please send your application (CV, a list of publications and two reference letters) by email to shashi@ncbs.res.in, patricia.bassereau@curie.fr, pierre.sens@curie.fr, madan@ncbs.res.in before 1 March 2019.

 

Candidates should not have more than 4 years of postdoctoral research experience. Candidates must be sponsored by a local host who is a member of NCBS/inStem/IC Faculty.

 

Deadlines

Stage 1: Joint Projects from the PI pairs accepted throughout the year

Stage 2: Applications from nominated postdoctoral candidates accepted by the academic office (acadoffice@ncbs.res.in) from 1st March 2019 - 31st March 2019.

Decisions about the short-listed candidates will be announced within 2 weeks after this deadline.

Intrviews and presentation by the short-listed candidates - First week of May 2019.

Please contact Johannes Ludger (Ludger.Johannes@curie.fr) or Raposo Graca (Graca.Raposo@curie.fr) at Institut Curie, Paris, France or Mukund Thattai (acadoffice@ncbs.res.in), Academic Office, inStem/NCBS, Bangalore, India for any additional information.