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COVID-19 Response: Local Logistics     National Effort

HTS & HCS Facility

 

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High Throughput and High Content Screening Facility

The HTS and HCS facility has evolved to assist academic and industrial researchers In developing and performing high-throughput and high content screens. The facility assist’s in the adaptation of both biochemical and cell based assays into the HTS/HCI  formats through miniaturization and automation.

The centre is equipped with state-of-the-art automated liquid handlers, multi-mode plate readers, automated imaging platforms, multiplexing system to quantify multiple analytes from single sample while housing a collection of RNAi libranes, small molecules and cell lines.

The facility supports biochemical and cellular assays through different readouts such as absorbance, fluorescence kinetics, fluorescence polarization, TR-FRET. Alpha Screen,  Alpha Lisa, bioluminescence and chemiluminescence.

The automated imaging platforms within the centre facilitates high content live and fixed cell imaging at better throughput owing to robotics and on-the-fly analysis. Some of the in-built analysis parameters include cell cycle analysis, cell spreading, cell motility, spot detection, etc.

In addition, the facility also hosts a BSL-2 Tissue Culture laboratory for mammalian and insect cell culture. In near future, the facility will add more instruments on automation and screening. Facility is developing new screening platforms such as small organism based screens,   ADME assays and 3D and Co culture systems.

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