In Vitro Anticancer Activity of Root's Extract toward Human Cervical Cancer and Identification of Potential Bioactive Compounds.
|Title||In Vitro Anticancer Activity of Root's Extract toward Human Cervical Cancer and Identification of Potential Bioactive Compounds.|
|Publication Type||Journal Article|
|Year of Publication||2021|
|Authors||Nayim P, Sudhir K, Mbaveng AT, Kuete V, Sanjukta M|
|Journal||Biomed Res Int|
is traditionally used to cure several diseases including cancer, wounds, and hypertension. The present study was designed to investigate the anticancer activity of the methanolic root extract of (IC-MeOH). The water-soluble tetrazolium-1 and colony formation assays were used to check the proliferation ability of the cells. Cell apoptosis and cell cycle were measured by flow cytometry-based fluorescence-activated cell sorting. The ultrahigh-performance liquid chromatography-high-resolution mass spectrometry (UHPLC-HRMS) analysis was used for the metabolites profiling of IC-MeOH. Based on high-mass accuracy, spectral data, and previous reports, tentative compound identifications were assigned. Our findings revealed that IC-MeOH inhibited the proliferation of HeLa and CaSki cells. The plant extract was also found to induce a concentration- and time-dependent apoptosis and cell cycle arrest in the G0/G1 phase (IC value) in CaSki cell line. Analysis of IC-MeOH permitted the identification of 10 compounds already reported for their anticancer activity, epicatechin, curcumin, (-)-yatein, caffeic acid, myricetin, jatrorrhizine, harmaline, cinnamaldehyde, dobutamine, and syringin. In conclusion, IC-MeOH is a rich source of cytotoxic metabolites that inhibits human cervical cancer proliferation apoptosis and cell cycle arrest.
|Alternate Journal||Biomed Res Int|
|PubMed Central ID||PMC8545510|