TitleUsing the folding landscapes of proteins to understand protein function.
Publication TypeJournal Article
Year of Publication2016
AuthorsRao VHemanth Gi, Gosavi S
JournalCurr Opin Struct Biol
Date Published2016 Feb

Proteins fold on a biologically-relevant timescale because of a funnel-shaped energy landscape. This landscape is sculpted through evolution by selecting amino-acid sequences that stabilize native interactions while suppressing stable non-native interactions that occur during folding. However, there is strong evolutionary selection for functional residues and these cannot be chosen to optimize folding. Their presence impacts the folding energy landscape in a variety of ways. Here, we survey the effects of functional residues on folding by providing several examples. We then review how such effects can be detected computationally and be used as assays for protein function. Overall, an understanding of how functional residues modulate folding should provide insights into the design of natural proteins and their homeostasis.

Alternate JournalCurr. Opin. Struct. Biol.
PubMed ID26812092