Two photon imaging of calcium responses in murine Purkinje neurons.
|Title||Two photon imaging of calcium responses in murine Purkinje neurons.|
|Publication Type||Journal Article|
|Year of Publication||2022|
|Authors||Dhanya SKumari, Hasan G|
|Date Published||2022 Mar 18|
Purkinje neurons (PNs) are an important component of the motor learning and coordination circuit and are affected in spino-cerebellar ataxias. Maintaining healthy PNs in cerebellar slices and recording their Ca transients can be challenging. Here, we describe a protocol for measuring Ca transients in PNs from adult mice, including problem-solving tips. This protocol can be used to measure neuronal excitability and agonist-mediated Ca signaling in cerebellar slices expressing a genetic Ca reporter in all PNs, thus improving yield of data. For complete details on the use and execution of this profile, please refer to Dhanya and Hasan (2021).
|Alternate Journal||STAR Protoc|
|PubMed Central ID||PMC8783150|