Two photon imaging of calcium responses in murine Purkinje neurons.
| Title | Two photon imaging of calcium responses in murine Purkinje neurons. |
| Publication Type | Journal Article |
| Year of Publication | 2022 |
| Authors | Dhanya SKumari, Hasan G |
| Journal | STAR Protoc |
| Volume | 3 |
| Issue | 1 |
| Pagination | 101105 |
| Date Published | 2022 Mar 18 |
| ISSN | 2666-1667 |
| Abstract | Purkinje neurons (PNs) are an important component of the motor learning and coordination circuit and are affected in spino-cerebellar ataxias. Maintaining healthy PNs in cerebellar slices and recording their Ca transients can be challenging. Here, we describe a protocol for measuring Ca transients in PNs from adult mice, including problem-solving tips. This protocol can be used to measure neuronal excitability and agonist-mediated Ca signaling in cerebellar slices expressing a genetic Ca reporter in all PNs, thus improving yield of data. For complete details on the use and execution of this profile, please refer to Dhanya and Hasan (2021). |
| DOI | 10.1016/j.xpro.2021.101105 |
| Alternate Journal | STAR Protoc |
| PubMed ID | 35098161 |
| PubMed Central ID | PMC8783150 |