TitleTwo photon imaging of calcium responses in murine Purkinje neurons.
Publication TypeJournal Article
Year of Publication2022
AuthorsDhanya SKumari, Hasan G
JournalSTAR Protoc
Date Published2022 Mar 18

Purkinje neurons (PNs) are an important component of the motor learning and coordination circuit and are affected in spino-cerebellar ataxias. Maintaining healthy PNs in cerebellar slices and recording their Ca transients can be challenging. Here, we describe a protocol for measuring Ca transients in PNs from adult mice, including problem-solving tips. This protocol can be used to measure neuronal excitability and agonist-mediated Ca signaling in cerebellar slices expressing a genetic Ca reporter in all PNs, thus improving yield of data. For complete details on the use and execution of this profile, please refer to Dhanya and Hasan (2021).

Alternate JournalSTAR Protoc
PubMed ID35098161
PubMed Central IDPMC8783150