A simple non-invasive technique for venom milking from a solitary wasp Delta conoideum Gmelin (Hymenoptera: Vespidae).
| Title | A simple non-invasive technique for venom milking from a solitary wasp Delta conoideum Gmelin (Hymenoptera: Vespidae). |
| Publication Type | Journal Article |
| Year of Publication | 2016 |
| Authors | Bhagavathula NChaitanya, Kumar M, Krishnappa C |
| Journal | Toxicon |
| Volume | 109 |
| Pagination | 4-6 |
| Date Published | 2016 Jan |
| ISSN | 1879-3150 |
| Abstract | Prospecting wasp, ant and bee venom for active bio-molecules has gained considerable interest among researchers in recent years. Collecting sufficient quantity of venom from solitary wasps without sacrificing them is often difficult. Here we describe a non-invasive technique for collecting venom from a solitary wasp Delta conoideum Gmelin (Red-backed potter wasp). Venom was milked by presenting an agar block to a single female wasp for stinging. The venom was extracted from the agar block using ACN: water solvent system. The total protein in venom was estimated quantitatively and the presence of peptides in the venom was confirmed by MALDI-TOF analysis. The proposed technique is non-invasive and pure venom can be repeatedly 'milked' using this method from other wasps and also bees without the need for sacrificing a large number of individuals. |
| DOI | 10.1016/j.toxicon.2015.10.023 |
| Alternate Journal | Toxicon |
| PubMed ID | 26556656 |