Prolongation of mitosis is associated with enhanced endogenous DNA damage in fission yeast.
|Title||Prolongation of mitosis is associated with enhanced endogenous DNA damage in fission yeast.|
|Publication Type||Journal Article|
|Year of Publication||2023|
|Authors||Jain I, Tran PT|
Mitosis is usually shorter than other phases of the cell cycle and maintains a consistent duration despite variations in cell size and spindle size. This suggests the existence of a compensatory mechanism that ensures a short duration, possibly as a protective measure against irreversible damage, such as DNA damage. To explore the link between prolonged mitosis and DNA damage, we develop a microscopy-based assay utilizing Rad52-GFP as a marker for mitotic DNA damage. Through this assay, we provide evidence that mutants with prolonged mitosis exhibit increased Rad52 puncta, indicating an elevation in endogenous DNA damage.
|Alternate Journal||MicroPubl Biol|
|PubMed Central ID||PMC10375284|