TitleProlongation of mitosis is associated with enhanced endogenous DNA damage in fission yeast.
Publication TypeJournal Article
Year of Publication2023
AuthorsJain I, Tran PT
JournalMicroPubl Biol
Date Published2023

Mitosis is usually shorter than other phases of the cell cycle and maintains a consistent duration despite variations in cell size and spindle size. This suggests the existence of a compensatory mechanism that ensures a short duration, possibly as a protective measure against irreversible damage, such as DNA damage. To explore the link between prolonged mitosis and DNA damage, we develop a microscopy-based assay utilizing Rad52-GFP as a marker for mitotic DNA damage. Through this assay, we provide evidence that mutants with prolonged mitosis exhibit increased Rad52 puncta, indicating an elevation in endogenous DNA damage.

Alternate JournalMicroPubl Biol
PubMed ID37521138
PubMed Central IDPMC10375284