Prolongation of mitosis is associated with enhanced endogenous DNA damage in fission yeast.
| Title | Prolongation of mitosis is associated with enhanced endogenous DNA damage in fission yeast. |
| Publication Type | Journal Article |
| Year of Publication | 2023 |
| Authors | Jain I, Tran PT |
| Journal | MicroPubl Biol |
| Volume | 2023 |
| Date Published | 2023 |
| ISSN | 2578-9430 |
| Abstract | Mitosis is usually shorter than other phases of the cell cycle and maintains a consistent duration despite variations in cell size and spindle size. This suggests the existence of a compensatory mechanism that ensures a short duration, possibly as a protective measure against irreversible damage, such as DNA damage. To explore the link between prolonged mitosis and DNA damage, we develop a microscopy-based assay utilizing Rad52-GFP as a marker for mitotic DNA damage. Through this assay, we provide evidence that mutants with prolonged mitosis exhibit increased Rad52 puncta, indicating an elevation in endogenous DNA damage. |
| DOI | 10.17912/micropub.biology.000911 |
| Alternate Journal | MicroPubl Biol |
| PubMed ID | 37521138 |
| PubMed Central ID | PMC10375284 |