TitleMitochondrial genetic diversity of pest and vector species, Frankliniella schultzei (Thripidae: Thripinae).
Publication TypeJournal Article
Year of Publication2024
AuthorsSingha D, Patidar A, Pal S, Tyagi K, Kumar V
JournalMol Biol Rep
Volume52
Issue1
Pagination55
Date Published2024 Dec 17
ISSN1573-4978
KeywordsAnimals, Australia, Bayes Theorem, DNA Barcoding, Taxonomic, DNA, Mitochondrial, Gene Flow, Genetic Variation, Haplotypes, India, Insect Vectors, Mitochondria, Phylogeny, Thysanoptera
Abstract

BACKGROUND: Frankliniella schultzei (Trybom) is a serious pest and a carrier of tospoviruses in major agricultural crops. This species is a historical and unresolved species complex that contains genetically different cryptic species across the globe.

METHODS AND RESULTS: DNA barcodes were generated from freshly collected specimens of F. schultzei from India and Australia using the sanger sequencing. Seventy-five COI sequences were generated from India and Australia. Moreover, 318 sequences were downloaded (India, Australia, Pakistan, and Africa) from the NCBI GenBank to explore the genetic diversity and phylogeny. The minimum and maximum mean interspecific distance between 393 sequences was found to be 7.97% and 21.50%, respectively. Bayesian and Neighbour joining clustering indicated the presence of five putative species within F. schultzei that had sympatry and allopatry. Moreover, 20 haplotypes and 140 polymorphic sites were identified. The African clade is unique; it does not share haplotypes with any other countries, suggesting it may represent the true F. schultzei. Haplotype network analysis showed shallow gene flow and deep genetic variation between the populations. Signatures of recent population history events were measured using Fu's Fs test and Tajima's D test. Morphometric analysis based on seven characters is also carried out.

CONCLUSION: Phylogeny and genetic distance revealed the presence of five putative species within F. schultzei. On the contrary, morphology does not unequivocally corroborate the phylogenetic results, as morphometric analysis showed overlap among these clades. To resolve F. schultzei species complex, whole genome-based sequencing data are very much necessitated.

DOI10.1007/s11033-024-10108-4
Alternate JournalMol Biol Rep
PubMed ID39688736
PubMed Central ID1609225
Grant ListCRG/2023/000498 / / Science and Engineering Research Board /