Measurement of Substrate SUMOylation in Real Time by Fluorescence Spectroscopy.
| Title | Measurement of Substrate SUMOylation in Real Time by Fluorescence Spectroscopy. |
| Publication Type | Journal Article |
| Year of Publication | 2025 |
| Authors | Puri A, Tripathi V, Das R |
| Journal | Methods Mol Biol |
| Volume | 2957 |
| Pagination | 159-168 |
| Date Published | 2025 |
| ISSN | 1940-6029 |
| Keywords | Humans, Protein Processing, Post-Translational, Small Ubiquitin-Related Modifier Proteins, Spectrometry, Fluorescence, Sumoylation |
| Abstract | Post-translational modifications, such as SUMOylation, play a crucial role in regulating various cellular processes. Traditionally, in vitro SUMOylation assays are carried out using techniques like SDS-PAGE gels and Western blotting, which are often time consuming. These informative methods, which quantitate the increase in substrate's molecular weight, can introduce potential errors and thus a need arises for a more efficient and accurate alternative. We propose a real-time detection method for SUMOylation that utilizes a fluorescence-tagged substrate, which has been recently developed by our group (Tripathi and Das, CP Protein Sci 101:e111, 2020). We believe this method has the potential to significantly enhance the efficiency and accuracy of SUMOylation assessment, thereby advancing our understanding of SUMO signaling and its function. |
| DOI | 10.1007/978-1-0716-4710-3_11 |
| Alternate Journal | Methods Mol Biol |
| PubMed ID | 40875121 |
| PubMed Central ID | 6095470 |