A locomotor assay reveals deficits in heterozygous Parkinson's disease model and proprioceptive mutants in adult .
|Title||A locomotor assay reveals deficits in heterozygous Parkinson's disease model and proprioceptive mutants in adult .|
|Publication Type||Journal Article|
|Year of Publication||2019|
|Authors||Aggarwal A, Reichert H, VijayRaghavan K|
|Journal||Proc Natl Acad Sci U S A|
|Date Published||2019 Nov 20|
Severe locomotor impairment is a common phenotype of neurodegenerative disorders such as Parkinson's disease (PD). models of PD, studied for more than a decade, have helped in understanding the interaction between various genetic factors, such as and PINK1, in this disease. To characterize locomotor behavioral phenotypes for these genes, fly climbing assays have been widely used. While these simple current assays for locomotor defects in mutants measure some locomotor phenotypes well, it is possible that detection of subtle changes in behavior is important to understand the manifestation of locomotor disorders. We introduce a climbing behavior assay which provides such fine-scale behavioral data and tests this proposition for the model. We use this inexpensive, fully automated assay to quantitatively characterize the climbing behavior at high parametric resolution in 3 contexts. First, we characterize wild-type flies and uncover a hitherto unknown sexual dimorphism in climbing behavior. Second, we study climbing behavior of heterozygous mutants of genes implicated in the fly PD model and reveal previously unreported prominent locomotor defects in some of these heterozygous fly lines. Finally, we study locomotor defects in a homozygous proprioceptory mutation ( ) known to affect fine motor control in Moreover, we identify aberrant geotactic behavior in mutants, thereby opening up a finer assay for geotaxis and its genetic basis. Our assay is therefore a cost-effective, general tool for measuring locomotor behaviors of wild-type and mutant flies in fine detail and can reveal subtle motor defects.
|Alternate Journal||Proc. Natl. Acad. Sci. U.S.A.|