TitleA genome engineering resource to uncover principles of cellular organization and tissue architecture by lipid signalling.
Publication TypeJournal Article
Year of Publication2020
AuthorsTrivedi D, Cm V, Bisht K, Janardan V, Pandit A, Basak B, H S, Ramesh N, Raghu P
JournalElife
Volume9
Date Published2020 Dec 15
ISSN2050-084X
Abstract

Phosphoinositides (PI) are key regulators of cellular organization in eukaryotes and genes that tune PI signalling are implicated in human disease mechanisms. Biochemical analyses and studies in cultured cells have identified a large number of proteins that can mediate PI signalling. However, the role of such proteins in regulating cellular processes and development in metazoans remains to be understood. Here we describe a set of CRISPR based genome engineering tools that allow the manipulation of each of these proteins with spatial and temporal control during metazoan development. We demonstrate the use of these reagents to deplete a set of 103 proteins individually in the eye and identify several new molecules that control eye development. Our work demonstrates the power of this resource in uncovering the molecular basis of tissue homeostasis during normal development and in human disease biology.

DOI10.7554/eLife.55793
Alternate JournalElife
PubMed ID33320085
Grant ListBT/PRJ3748/GET/l 19/27/2015 / / Department of Biotechnology, Ministry of Science and Technology, India /
IA/S/14/2/501540 / / Wellcome-DBT India Alliance /
core / / National Centre for Biological Sciences /
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