TitleFast, Efficient, and Stable Conjugation of Multiple DNA Strands on Colloidal Quantum Dots.
Publication TypeJournal Article
Year of Publication2015
AuthorsBanerjee A, Grazon C, Nadal B, Pons T, Krishnan Y, Dubertret B
JournalBioconjug Chem
Date Published2015 Aug 19

A novel method for covalent conjugation of DNA to polymer coated quantum dots (QDs) is investigated in detail. This method is fast and efficient: up to 12 DNA strands can be covalently conjugated per QD in optimized reaction conditions. The QD-DNA conjugates can be purified using size exclusion chromatography and the QDs retain high quantum yield and excellent stability after DNA coupling. We explored single-stranded and double-stranded DNA coupling, as well as various lengths. We show that the DNA coupling is most efficient for short (15 mer) single-stranded DNA. The DNA coupling has been performed on QDs emitting at four different wavelengths, as well as on gold nanoparticles, suggesting that this technique can be generalized to a wide range of nanoparticles.

Alternate JournalBioconjug. Chem.
PubMed ID25992903