Extraction and Purification of Laccases from Rice Stems.
Title | Extraction and Purification of Laccases from Rice Stems. |
Publication Type | Journal Article |
Year of Publication | 2019 |
Authors | Swetha C, Shivaprasad PV |
Journal | Bio Protoc |
Volume | 9 |
Issue | 7 |
Pagination | e3208 |
Date Published | 2019 Apr 05 |
ISSN | 2331-8325 |
Abstract | Laccases are found in cell walls of plants in very low amounts. This protocol provides an efficient method to purify laccases from rice stems. The method involves three steps: 1) Isolation of total protein from rice stems using buffers with high salt concentration to extract protein from cell walls; 2) Purification of laccases using concanavalin-A beads; and, 3) In-gel staining of laccases with 4-hydroxyindole. Concanavalin-A specifically binds to internal or non-reducing terminal α-D-mannosyl and α-D-glucosyl groups found in glycoproteins and glycolipids. Laccases being glycoproteins binds to concanavalin-A during purification process and eluted with mannose. |
DOI | 10.21769/BioProtoc.3208 |
Alternate Journal | Bio Protoc |
PubMed ID | 33655003 |
PubMed Central ID | PMC7854116 |