TitleA DNA Aptamer for Cyclic Adenosine Monophosphate that Shows Adaptive Recognition.
Publication TypeJournal Article
Year of Publication2020
AuthorsSharma S, Zajac M, Krishnan Y
Date Published2019 May 17

As a ubiquitous second messenger, cyclic adenosine monophosphate (cAMP) mediates diverse biological processes such as cell growth, inflammation, and metabolism. The ability to probe these pathways would be significantly enhanced if we had a DNA-based sensor for cAMP. Herein, we describe a new, 31-base long single-stranded DNA aptamer for cAMP, denoted caDNApt-1, that was isolated by in vitro selection using systemic evolution of ligands after exponential enrichment (SELEX). caDNApt-1 has an approximately threefold higher affinity for cAMP than ATP, ADP, and AMP. Using non-denaturing gel electrophoresis and fluorescence spectroscopy, we characterized the structural changes caDNApt-1 undergoes upon binding to cAMP and revealed its potential as a cAMP sensor.

Alternate JournalChembiochem
PubMed ID31099939
Grant List500095MA / / The Wellcome Trust DBT India Alliance /