CRISPR-Cas-Induced Mutants Identify a Requirement for dSTIM in Larval Dopaminergic Cells of Drosophila melanogaster.
Title | CRISPR-Cas-Induced Mutants Identify a Requirement for dSTIM in Larval Dopaminergic Cells of Drosophila melanogaster. |
Publication Type | Journal Article |
Year of Publication | 2017 |
Authors | Pathak T, Trivedi D, Hasan G |
Journal | G3 (Bethesda) |
Volume | 7 |
Issue | 3 |
Pagination | 923-933 |
Date Published | 2017 Mar 10 |
ISSN | 2160-1836 |
Abstract | Molecular components of store-operated calcium entry have been identified in the recent past and consist of the endoplasmic reticulum (ER) membrane-resident calcium sensor STIM and the plasma membrane-localized calcium channel Orai. The physiological function of STIM and Orai is best defined in vertebrate immune cells. However, genetic studies with RNAi strains in Drosophila suggest a role in neuronal development and function. We generated a CRISPR-Cas-mediated deletion for the gene encoding STIM in Drosophila (dSTIM), which we demonstrate is larval lethal. To study STIM function in neurons, we merged the CRISPR-Cas9 method with the UAS-GAL4 system to generate either tissue- or cell type-specific inducible STIM knockouts (KOs). Our data identify an essential role for STIM in larval dopaminergic cells. The molecular basis for this cell-specific requirement needs further investigation. |
DOI | 10.1534/g3.116.038539 |
Alternate Journal | G3 (Bethesda) |
PubMed ID | 28131984 |
PubMed Central ID | PMC5345722 |
Grant List | P40 OD018537 / OD / NIH HHS / United States |