Monitoring protein ubiquitination and SUMOylation in real-time by NMR.
| Title | Monitoring protein ubiquitination and SUMOylation in real-time by NMR. |
| Publication Type | Journal Article |
| Year of Publication | 2020 |
| Authors | Habibullah BIsmail, Tripathi V, Surana P, Das R |
| Journal | Chem Commun (Camb) |
| Volume | 56 |
| Issue | 49 |
| Pagination | 6735-6738 |
| Date Published | 2020 Jun 18 |
| ISSN | 1364-548X |
| Abstract | The covalent conjugation of ubiquitin (Ub), known as ubiquitination, is a multi-step reaction involving multiple enzymes. We report a real-time, tag-free method to monitor protein ubiquitination by NMR spectroscopy under physiological conditions. The approach is also applicable for monitoring other ubiquitin-like modifications, and the disassembly of Ub polymers. |
| DOI | 10.1039/d0cc02252g |
| Alternate Journal | Chem. Commun. (Camb.) |
| PubMed ID | 32426760 |