Method/Protocol developed by facility
 
1) T Naik, M Sharda, Lakshminarayanan CP, K Virbhadra, A Pandit* (2023) High-quality single amplicon sequencing method for illumina MiSeq platform using pool of ‘N’(0–10) spacer-linked target specific primers without PhiX spike-in BMC genomics 24 (1), 1-11A https://doi.org/10.1186/s12864-023-09233-4
 
This innovative method helps:
 
a)  Boosting base diversity at each sequencing cycle
 
b)  Producing high-quality reads
 
c)  Eliminates the need for PhiX spike-in for single amplicon sequencing on illumina platform
 
d) Per sample 30-40% cost reduction for single amplicon sequencing e.g. 16S (V3-V4 region)/ITS/18S 
 
2) JK Dhall*, N Kasturacharya, A Pandit*, CP Lakshminarayanan (2023) Optimized protocol for assay for transposase-accessible chromatin by sequencing (ATAC-seq) from Drosophila melanogaster brain tissue STAR protocols 4 (2), 102153 https://doi.org/10.1016/j.xpro.2023.102153
 
a) We have provided a detailed protocol of ATAC-seq assay from Drosophila brain tissue. 
 
b) Starting from dissection and transposition to amplification of libraries has been elaborated.