Course :  Biomolecular labelling and imaging

Course details: This course covers the chemical strategies for labelling biomolecules, focusing on the design principles and pitfalls of chemical probes for biological labelling and imaging. Students will learn about reactive sites on biomolecules, site-specific conjugation, and advanced labelling techniques like click chemistry. The course emphasizes the chemistry behind biomolecular imaging tools used to study proteins, nucleic acids, lipids, and other biomolecules.

Topics:

Biomolecules of life and reactive centers on biomolecules

Basics of fluorescence spectroscopy

Photochemistry of fluorophores

Fluorescence and environmental sensitivity

Protein labelling

Protein imaging

Click labelling and imaging

DNA/RNA labelling

DNA/RNA imaging

Lipid labelling

Lipid imaging

Glycan labelling

Glycan imaging

Metabolite imaging

Other imaging techniques: Raman imaging, IR imaging

Key papers in chemistry that changed modern biological labelling and imaging

Reference Books: Principles of fluorescence spectroscopy by Joseph R. Lakowicz