Targeted Bisulfite Sequencing and DNA Methylation Analysis in Rice Endosperm and Other Tissues.
| Title | Targeted Bisulfite Sequencing and DNA Methylation Analysis in Rice Endosperm and Other Tissues. |
| Publication Type | Journal Article |
| Year of Publication | 2026 |
| Authors | Pal AKumar, Gandhivel VHari-Sunda, Shivaprasad PV |
| Journal | Methods Mol Biol |
| Volume | 2988 |
| Pagination | 71-82 |
| Date Published | 2026 |
| ISSN | 1940-6029 |
| Keywords | DNA Methylation, DNA, Plant, Endosperm, Epigenesis, Genetic, Genome, Plant, High-Throughput Nucleotide Sequencing, Oryza, Polymerase Chain Reaction, Sequence Analysis, DNA, Sulfites |
| Abstract | DNA methylation is a major epigenetic mark on chromatin. Tissue-specific DNA methylation in the regulatory regions of key genes modulates their tissue-specific expression. To study DNA methylation levels at specific sequence contexts across tissues or during various environmental conditions, whole-genome DNA methylation analysis is widely used. However, whole-genome methylation analysis is an expensive, time-consuming technique and requires specific skills. To understand the role of DNA methylation in a small number of candidate loci, targeted bisulfite (BS) PCR-sequencing analysis is an attractive option. Here, we describe a detailed method to understand DNA methylation by targeted-BS PCR analysis using rice endosperm tissues. This method can be readily extended to multiple monocots, across various tissues and various stress conditions. |
| DOI | 10.1007/978-1-0716-4981-7_7 |
| Alternate Journal | Methods Mol Biol |
| PubMed ID | 41051667 |
| PubMed Central ID | 4292151 |