Patterned Optogenetic Stimulation Using a DMD Projector.
|Title||Patterned Optogenetic Stimulation Using a DMD Projector.|
|Publication Type||Book Chapter|
|Year of Publication||2021|
|Authors||Bhatia A, Moza S, Bhalla US|
|Series Volume||Methods Mol Biol|
Optical manipulation is a powerful way to control neural activity in vitro and in vivo with millisecond precision. Patterning of light provides the remarkable ability to simultaneously target spatially segregated neurons from a population. Commercially available projectors provide one of the simplest and most economical ways of achieving spatial light modulation at millisecond timescales. Here, we describe the protocol for constructing a projector-based spatio-temporal light patterning system integrated with a microscope on a typical electrophysiology rig. The set-up is well suited for applications requiring rapid, distinct, and combinatorial inputs, akin to brain activity. This equipment involved is fairly economical (<$5000 including all optical and mechanical components), and the set-up is easy to assemble and program.
|Abbreviation||Methods Mol. Biol.|