Monitoring protein ubiquitination and SUMOylation in real-time by NMR.
|Title||Monitoring protein ubiquitination and SUMOylation in real-time by NMR.|
|Publication Type||Journal Article|
|Year of Publication||2020|
|Authors||Habibullah BIsmail, Tripathi V, Surana P, Das R|
|Journal||Chem Commun (Camb)|
|Date Published||2020 Jun 18|
The covalent conjugation of ubiquitin (Ub), known as ubiquitination, is a multi-step reaction involving multiple enzymes. We report a real-time, tag-free method to monitor protein ubiquitination by NMR spectroscopy under physiological conditions. The approach is also applicable for monitoring other ubiquitin-like modifications, and the disassembly of Ub polymers.
|Alternate Journal||Chem. Commun. (Camb.)|