TitleMonitoring protein ubiquitination and SUMOylation in real-time by NMR.
Publication TypeJournal Article
Year of Publication2020
AuthorsHabibullah BIsmail, Tripathi V, Surana P, Das R
JournalChem Commun (Camb)
Volume56
Issue49
Pagination6735-6738
Date Published2020 Jun 18
ISSN1364-548X
Abstract

The covalent conjugation of ubiquitin (Ub), known as ubiquitination, is a multi-step reaction involving multiple enzymes. We report a real-time, tag-free method to monitor protein ubiquitination by NMR spectroscopy under physiological conditions. The approach is also applicable for monitoring other ubiquitin-like modifications, and the disassembly of Ub polymers.

DOI10.1039/d0cc02252g
Alternate JournalChem. Commun. (Camb.)
PubMed ID32426760