TitleInteractions Between Epidermal Keratinocytes, Dendritic Epidermal T-Cells, and Hair Follicle Stem Cells.
Publication TypeJournal Article
Year of Publication2018
AuthorsBadarinath K, Dutta A, Hegde A, Pincha N, Gund R, Jamora C
JournalMethods Mol Biol
Date Published2018 Jun 13
ISSN1940-6029
Abstract

The interplay of immune cells and stem cells in maintaining skin homeostasis and repair is an exciting new frontier in cutaneous biology. With the growing appreciation of the importance of this new crosstalk comes the requirement of methods to interrogate the molecular underpinnings of these leukocyte-stem cell interactions. Here we describe how a combination of FACS, cellular coculture assays, and conditioned media treatments can be utilized to advance our understanding of this emerging area of intercellular communication between immune cells and stem cells.

DOI10.1007/7651_2018_155
Alternate JournalMethods Mol. Biol.
PubMed ID29896657