Immunofluorescence Staining and Microscopic Imaging of Plant Nuclei for Epigenetic Modifications.
| Title | Immunofluorescence Staining and Microscopic Imaging of Plant Nuclei for Epigenetic Modifications. |
| Publication Type | Journal Article |
| Year of Publication | 2026 |
| Authors | Gandhivel VHari-Sunda, Raju S, Shivaprasad PV |
| Journal | Methods Mol Biol |
| Volume | 2988 |
| Pagination | 83-92 |
| Date Published | 2026 |
| ISSN | 1940-6029 |
| Keywords | Cell Nucleus, Chromatin, DNA Methylation, Epigenesis, Genetic, Epigenomics, Fluorescent Antibody Technique, Histones, Microscopy, Fluorescence, Plants, Protein Processing, Post-Translational |
| Abstract | Histone posttranslational modifications (PTMs) and DNA methylation are the predominant epigenetic modifications on the chromatin that regulate gene expression. These modifications can be spatially resolved using microscopic examination of the nuclei with the help of commercially available antibodies. Here, we describe a detailed method to obtain intact nuclei from plant tissues and reproducibly immunostain the nuclei for specific chromatin marks and microscopic examination. This method can be readily extended to multiple plant species as the antibodies are raised against conserved epigenetic marks. |
| DOI | 10.1007/978-1-0716-4981-7_8 |
| Alternate Journal | Methods Mol Biol |
| PubMed ID | 41051668 |
| PubMed Central ID | 10980423 |