Course Schedule
Sunday, Sept. 15: Opening Lecture, Poster Session |
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12:30-01:30pm |
Lunch Location: Glass roof top canteen |
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01:30-02:00pm |
Participants Registration Location: Reception, Southern Lab Complex (SLC) |
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02:00-03:15pm |
Opening Remarks: Satyajit Mayor, National Centre for Biological Sciences - TIFR, Bangalore Location: Dasheri (SLC) |
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Research Lecture 1: Jan Ellenberg, European Molecular Biology Laboratory, Heidelberg, Germany Location: Dasheri (SLC) |
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03:15-03:30pm |
Coffee Break Location: Outside Dasheri (SLC) |
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03:30-04:30pm |
Didactic Lecture 1: Basics of Microscopy, Köhler illumination and Resolution, Diffraction. - Manoj Mathew, Carl Zeiss, Singapore Location: Raspuri (New Teaching Lab) |
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04:30-08:00pm |
Poster session by the students attending the course & Social Location: SLC Atrium |
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08:00-09:00pm |
Dinner Location: Glass roof top canteen |
Monday, Sept. 16: Basics of microscopy: Microscope Light Path, Diffraction/Resolution, Phase and Polarization |
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08:30-09:30am |
Didactic Lecture 2: Contrast enhancement -Phase Contrast, Dark Field, Polarized Light, DIC - Eric Griffis, University of California - San Diego, USA Location: Raspuri (New Teaching Lab) |
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09:30am-03:15pm |
Laboratory 1: Microscopy light path, Köhler illumination on rails Introduction to Transmitted Light Microscopy, Köhler Illumination and Resolution, Identify Parts of a Microscope and Light Paths, Köhler Illumination on Stained Histology Slides, Exercise to Look at Resolution/Diffraction with Diatoms Samples: Bead Slides, Diatoms, Pond Water Lab leader: Manoj Mathew, Carl Zeiss, Singapore Lab Instructors: Anjana Badrinarayanan, Eric Griffis, Gautam Soni, Jonathan Ashmore, Minhaj Sirajuddin, Rahul Roy, Thomas van Zanten, and Company Application Specialists Location: Raspuri (New Teaching Lab) |
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11:00-11:15am |
Coffee Break Location: Outside Raspuri (New Teaching Lab) |
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12:30-01:15pm |
Lunch Location: Glass roof top canteen |
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03:15-04:15pm |
Didactic Lecture 3: Practical Aspects of Objective Lens Design, covering lenses and aberrations - Eric Griffis, University of California - San Diego, USA Location: Raspuri (New Teaching Lab) |
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04:15-04:30pm |
Coffee Break Location: Outside Raspuri (New Teaching Lab) |
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04:30-06:30pm |
Laboratory 2: Phase Contrast, DIC and Dark Field Microscopy Lab leader: Eric Griffis, University of California - San Diego, USA Lab Instructors: Anjana Badrinarayanan, Jonathan Ashmore, Manoj Mathew, Minhaj Sirajuddin, Rahul Roy, Thomas van Zanten, and Company Application Specialists Location: Raspuri (New Teaching Lab) |
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06:30-07:30pm |
Didactic Lecture 4: Principles of Fluorescence and Fluorescence microscopy - Thomas van Zanten, National Centre for Biological Sciences - TIFR, Bangalore Location: Raspuri (New Teaching Lab) |
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07:30-08:30pm |
Dinner Location: Glass roof top canteen |
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08:30-09:30pm |
Didactic Lecture 5: Detectors - Manoj Mathew, Carl Zeiss, Singapore |
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09:30-11:00pm |
Free time on Microscopes |
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Tuesday, Sept. 17: Fluorescence Microscopy |
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08:30-09:30am |
Didactic Lecture 6: Fluorescent dyes, Fluorescent Proteins and Selection of Fluorescent Probes - Rahul Roy, Indian Institute of Science, Bangalore Location: Raspuri (New Teaching Lab) |
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09:30-11:30am |
Laboratory 3: Fluorescence Lab A: Examine the fluorescence light path and dichroics/filters. Take images of fluorescence fixed specimens, Fluorescence beads for PSF and determining pixel shift Lab leader: Rahul Roy, Indian Institute of Science, Bangalore Lab Instructors: Anjana Badrinarayanan, Eric Griffis, Gautam Soni, Jonathan Ashmore, Manoj Mathew, Minhaj Sirajuddin, Thomas van Zanten, and Company Application Specialists Location: Raspuri (New Teaching Lab) |
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11:30-11:45am |
Coffee Break Location: Outside Raspuri (New Teaching Lab) |
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11:45-12:45pm |
Didactic Lecture 7: Image Analysis - Julius Hossain, European Molecular Biology Laboratory, Germany Location: Raspuri (New Teaching Lab) |
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12:45-01:30pm |
Lunch Location: Glass roof top canteen |
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01:30-03:30pm |
Laboratory 4: Fluorescence Lab B: Fluorescence, Time Lapse, Cameras and projections of PSFs, Multi-wavelength Time Lapse Imaging of living cells, Measure read-out noise and photon conversion factor of the camera. Lab leader: Rahul Roy, Indian Institute of Science, Bangalore Lab Instructors: Anjana Badrinarayanan, Eric Griffis, Jonathan Ashmore, Manoj Mathew, Minhaj Sirajuddin, Thomas van Zanten, and Company Application Specialists Location: Raspuri (New Teaching Lab) |
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03:30-03:45pm |
Coffee Break Location: Outside Dasheri (SLC) |
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03:45-04:30pm |
Research Lecture 2: Satyajit Mayor, National Centre for Biological Sciences - TIFR, Bangalore Location: Dasheri (SLC) |
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04:30-05:15pm |
Research Lecture 3: Jonathan Ashmore, University College London, UK Location: Dasheri (SLC) |
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05:15-05:30pm |
Coffee Break Location: Outside Raspuri (New Teaching Lab) |
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05:30-06:30pm |
Didactic Lecture 8: Optical Sectioning Techniques- Confocal Microscopy - Sudipta Maiti, Tata Institute of Fundamental Research, Mumbai Location: Raspuri (New Teaching Lab) |
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06:30-08:00pm |
Free Time on Microscopes |
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06:30-10:00pm |
Dinner (For Faculty- Down Town Bangalore) |
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08:00-09:00pm |
Dinner (For Participants, Others - Glass roof top canteen) |
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09:00-11:00pm |
Free time on microscopes |
Wednesday, Sept. 18: Optical Sectioning and Enhancing Resolution |
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08:30am-12:45pm |
Laboratory 5: Optical Sectioning Techniques (point scanning, spinning disk) 2 x 2hr modules Lab Leader: H. Krishnamurthy, NCBS, India
1. Equipment: Olympus FV3000 point scanning Microscope - Upright Location: CIFF, Southern Laboratories Complex (SLC) Lab Instructor: Olympus Application Specialist
2. Equipment: Olympus FV3000 point scanning Microscope - Inverted Location: CIFF, Southern Laboratories Complex (SLC) Lab Instructor: Olympus Application Specialist
3. Equipment: Leica SP5 point scanning microscope Location: CIFF, Southern Laboratories Complex (SLC) Lab Instructor: Leica Application Specialist
4. Equipment: Andor spinning disk microscope Location: Satyajit Mayor Lab, Southern Laboratories Complex (SLC) Lab Instructor: Sowmya Jahnavi Chatty
5. Equipment: Zeiss LSM 800 NLO point scanning microscope Location: CIFF, Southern Laboratories Complex (SLC) Lab Instructor: Zeiss Application Specialist
6. Equipment: Nikon A1RHD 25 Confocal Microscope Location: CIFF, Southern Laboratories Complex (SLC) Lab Instructor: Nikon Application Specialist |
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10:30-10:45am |
Coffee Break Location: Outside Dasheri (SLC) |
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12:45-02:00pm |
Discussion over Lunch: Small group discussions of students with faculty over lunch about their Research/Microscopy interests Location: Glass roof top canteen |
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02:00-03:00pm |
Didactic Lecture 9: Fluorescence Microscopy based methods to study protein dynamics in live cells: from FRAP to FCS - Sudipta Maiti, Tata Institute of Fundamental Research, Mumbai Location: Raspuri (New Teaching Lab) |
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03:00-04:00pm |
Didactic Lecture 10: FRET Microscopy- Satyajit Mayor, National Centre for Biological Sciences - TIFR, Bangalore. Location: Raspuri (New Teaching Lab) |
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04:00-04:15 pm |
Coffee Break Location: Outside Dasheri (SLC) |
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04:15-05:00 pm |
Research Lecture 4: Sudipta Maiti, Tata Institute of Fundamental Research, Mumbai Location: Dasheri (SLC) |
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05:00-06:15pm |
Award ceremony Location: Dasheri (SLC) |
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06:15-7:00pm |
Dinner Location: Glass roof top canteen |
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07:00-10:00pm |
Cultural and Social Event Location: inStem Atrium |
Thursday, Sept. 19: Special Topics |
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08:30-09:30am |
Didactic Lecture 11: TIRF, RICM - Bidisha Sinha, Indian Institute of Science Education and Research, Kolkata Location: Raspuri (New Teaching Lab) |
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09:30-10:30am |
Didactic Lecture 12: Super-resolution Microscopy - STED - María García-Parajo, ICFO, Spain Location: Raspuri (New Teaching Lab) |
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10:30-10:45am |
Coffee Break Location: Outside Raspuri (New Teaching Lab) |
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10:45am-02:45pm |
Laboratory 6: Specialized techniques 2 x 1.5hr modules, distributed by student signup and selection. There will be 2 rotations of 2 hour each. Students can choose in advance and a rotation plan will be prepared.
Technique 1: Integrating microscope systems and Configuring Automation Software for Microscope Equipment: Nikon Ti2-E Lab Instructor: Eric Griffis Location: Raspuri (New Teaching Lab)
Technique 2: Homo-Försters Resonance Energy Transfer (Homo-FRET) Equipment: Nikon TIRF microscope with dual camera Lab Instructor: Sarayu Beri Location: Mayor Lab, Southern Laboratories Complex (SLC)
Technique 3: Laser Micro Dissection and Catapulting system Equipment: Zeiss PALM Microbeam Laser microdissection microscope Lab Instructor: Zeiss Application Specialist Location: CIFF, Southern Laboratories Complex (SLC)
Technique 4: TIRF Microscopy Equipment: Nikon TIRF microscope with dual camera Lab Instructor: Sankarshan Talluri Location: Mayor Lab, Southern Laboratories Complex (SLC)
Technique 5: SIM and STORM Super Resolution Microscope Equipment: Nikon N-SIM/N-STORM Lab Instructor: Nikon Application Specialist Location: CIFF, Southern Laboratories Complex (SLC)
Technique 6: Fluorescence Recovery After Photobleaching(FRAP) Equipment: Olympus FV3000 Lab Instructor: Olympus Application Specialist Location: CIFF, Southern Laboratories Complex (SLC)
Technique 7: Airyscanning Super Resolution Confocal Microscopy Equipment: Zeiss LSM800 Lab Instructor: Zeiss Application Specialist Location: CIFF, Southern Laboratories Complex (SLC) |
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12:15- 01:15pm |
Lunch Location: Glass roof top canteen |
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02:45-03:00pm |
Coffee Break Location: Outside Dasheri (SLC) |
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03:00-03:45pm |
Research Lecture 5: Bidisha Banerjee Sinha, IISER, Kolkata Location: Dasheri (SLC) |
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03:45-04:00pm |
Coffee Break Location: Glass roof top canteen |
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04:00-06:00pm |
Technical Presentations by sponsors Location: Haapus (LH1, Eastern Lab Complex) |
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06:00-07:00pm |
Didactic Lecture 13: Single Particle Imaging and Tracking - María García-Parajo, ICFO, Spain Location: Raspuri (New Teaching Lab) |
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07:00-08:00pm |
Dinner Location: Glass roof top canteen |
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08:00-10:00pm |
Laboratory 7: Image Processing/Analysis Workshop Lab leader: Julius Hossain, European Molecular Biology Laboratory, Germany Lab Instructors: Lakshmi B. Location: Raspuri (New Teaching Lab) |
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10:00-11:00pm |
Free Time on Microscopes |
Friday, Sept. 20: Specialized Techniques |
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08:30-09:30am |
Didactic Lecture 14: SIM/PALM/STORM - Adish Dani, TIFR Centre for Interdisciplinary Studies, Hyderabad Location: Raspuri (New Teaching Lab) |
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09:30- 10:30am |
Didactic Lecture 15: Optical Tweezers - Gautam Vivek Soni, Raman Reserach Institute, Bangalore Location: Raspuri (New Teaching Lab) |
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10:30-10:45am |
Coffee Break Location: Outside Raspuri (New Teaching Lab) |
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10:45am-03:45pm |
Laboratory 6: (continued) Specialized techniques, 2 x 1.5hr modules, distributed by student signup and selection. There will be 2 rotations of 1.5 hours each. Students can choose in advance and a rotation plan will be prepared. |
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12:15-2:15pm |
Discussion over Lunch: Small group discussions of students with faculty over lunch about their Research/Microscopy interests Location: Glass roof top canteen |
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03:45-04:00pm |
Coffee Break Location: Outside Dasheri (SLC) |
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04:00-04:45pm |
Research Lecture 6: María García-Parajo, ICFO, Spain Location: Dasheri (SLC) |
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04:45-05:30pm |
Research Lecture 7: Adish Dani, TIFR Centre for Interdisciplinary Sciences, Hyderabad Location: Dasheri (SLC) |
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05:30-06:00pm |
Group Photo |
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06:00-07:00pm |
Graduation Location: Dasheri (SLC) |
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07:00-08:00pm |
Dinner Location: Glass roof top canteen |
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08:00-11:00pm |
Free Time on Microscopes |
Saturday, Sept. 21: Projects |
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09:00-09:45am |
Research Lecture 8: Vinothkumar Kutti Ragunath, National Centre for Biological Sciences - TIFR, Bangalore Location: Dasheri (SLC) |
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09:45-10:30am |
Research Lecture 9: Rahul Roy, Indian Institute of Science, Bangalore Location: Dasheri (SLC) |
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10:30am-06:15pm |
Projects |
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01:00-01:45pm |
Lunch |
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06:15-10:00pm |
Workshop Dinner and Social (Downtown Bangalore) |
Sunday, Sept. 22: Projects |
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09:00am-03:45pm |
Projects |
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01:00-01:45pm |
Lunch |
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03:45-04:00pm |
Coffee Break Location: Glass roof top canteen |
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04:00-06:00pm |
Project Presentation by Participants Location: Raspuri (New Teaching Lab) |
