Course Schedule 2024
Course Schedule
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Sunday, Sept 22nd: Opening Lecture, Poster Session
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12:00-13:00 |
Participants Registration Location: Reception, Southern Laboratories Complex (SLC) |
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13:00-14:00 |
Lunch Location: NCBS Canteen 1st floor |
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14:00-14:30 |
Opening Remarks: Satyajit Mayor Location: Dasheri |
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14:30 – 15:15 |
Research Lecture 1: Satyajit Mayor, University of Warwick and NCBS, Bangalore Location: Dasheri |
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15:15-15:30 |
Coffee Break Location: Outside Dasheri |
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15:30-16:30 |
Didactic Lecture 1: Basics of Microscopy, Kohler illumination, Diffraction, Resolution, and Aberrations – Jennifer Ross, Syracuse University, USA Location: Dasheri |
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16:30-17:00 |
Group Photo Location: Lawn Outside Dasheri |
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17:00-18:00 |
Didactic Lecture 2: Contrast Enhancement – Phase contrast, Dark Field, Polarized Light, DIC – Jennifer Ross Location: Dasheri |
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18:00 – 20:00 |
Poster Session by the students attending the course Location: SLC Colonnade |
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20:00 – 21:00 |
Dinner Location: NCBS Canteen 1st Floor |
Monday, Sept 23rd: Basics of microscopy, Microscope Light path, Diffraction, Resolution, Phase and Polarization
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8:30-13:30 |
Laboratory 1: Microscopy and Kohler Illumination on rails Lab Leader: Jennifer Ross Lab Instructors: Robert Cross, Nicholas Carter, Rahul Roy, Satyajit Mayor Lab Support: Raman S, Piyush Chajjed, Palak Chugh, Mona Hosny, Fenil Ganatra, Neelanjana Ray, Victor Samuel, Nivya Mendon Location: Raspuri |
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11:00-11:15 |
Coffee Break Location: Outside Raspuri |
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13:30-14:30 |
Lunch Location: NCBS Canteen1st floor |
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14:30 – 15:30 |
Didactic Lecture 3: Principles of Fluorescence Microscopy-Fluorescent dyes and proteins, and selection of Fluorescent Probes) – Robert Cross Location: Haapus (LH1) |
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15:30-16:30 |
Didactic Lecture 4: Detectors and Cameras in Microscopy – Rahul Roy Location: Haapus (LH1) |
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16:30: 17:00 |
Coffee Break Location: Outside Raspuri |
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17:30-19:30 |
Laboratory 2: Phase contrast, DIC and Dark Field Microscopy Lab Leader: Jennifer Ross Lab Instructors: Minhaj Sirajuddin, Mahipal Ganji, Thorsten Wohland, Suliana Manley, Arjun Guha, Kedar Khare Lab Support: Raksha K, Vishwesh Haricharan Rai, Palak Chugh, Victor Samuel, Fenil Ganatra, Atal Vats, & Microscope Application Scientists Location: Raspuri |
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19:30 – 20:30 |
Dinner Location: NCBS Canteen 1st Floor |
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20:30 – 23:00 |
Free time on Microscopes |
Tuesday, Sept 24th: Fluorescence Microscopy
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8:30-11:30 |
Laboratory 3: Fluorescence Lab A: Examine the fluorescence light path and dichroic mirrors/ filters Lab Leader: Robert Cross Lab Instructors: Anjana Badarinarayanan, Nicholas Carter, Suliana Manley, Pramod Pullarkat, Minhaj Sirajuddin, Satyajit Mayor Lab Support: Nivya Mendon, Atal Vats, Shivangi Pandey, Sukanta Behera, Chandana S, Pooja S Krishna, & Microscope Application Specialists Location: Raspuri |
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11:30-11:45 |
Coffee Break |
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11:45 -12:45 |
Didactic Lecture 5: Optical Sectioning Techniques – Confocal Microscopy – Mahipal Ganji Location: Haapus (LH1) |
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12:45-13:30 |
Didactic Lecture 5: Total Internal Reflection Fluorescence (TIRF) Microscopy – Minhaj Sirajuddin Location: Haapus (LH1) |
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13:30 – 14:30 |
Lunch Location: NCBS Canteen 1st Floor |
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14:30-17:30 |
Laboratory 4: Fluorescence Lab B - Fluorescence, Time Lapse, Cameras and Z Projections of PSF Lab Leader: Rahul Roy Lab Instructors: Jennifer Ross, Mahipal Ganji, Arjun Guha, Thorsten Wohland, Pramod Pullarkat, Anjana Badarinarayanan Lab Support: Nivya Mendon, Atal Vats, Shivangi Pandey, Sukanta Behera, Raksha K, Pooja S Krishna, & Microscope Application Specialists Location: Raspuri |
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17:30 – 17:45 |
Coffee Break Location: Outside Raspuri |
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17:45 – 18:30 |
Research Lecture 2: Jennifer Ross, Syracuse University, USA Location: Dasheri |
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18:30 – 19:30 |
Didactic Lecture 6: Image Processing – Robert Cross and Nicholas Carter Location: Dasheri |
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20:00 – 22:00 |
Dinner (for Faculty – Down Town Bangalore) |
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20:00 – 21:00 |
Dinner (for Participants – NCBS Canteen First floor) |
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21:00 – 23:00 |
Free time on Microscopes |
Wednesday, Sept 25th: Optical Sectioning and Enhancing Resolution
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8:30-9:30 |
Didactic Lecture 7: FRET Microscopy – Satyajit Mayor Location: Haapus (LH1) |
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9:30-10:30 |
Didactic Lecture 8: Super-resolution Microscopy – PALM/STORM - Suliana Manley Location: Haapus (LH1) |
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10: 30 – 10:45 |
Coffee Break Location: Outside Haapus (LH1) |
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10:45 -15:30 |
Laboratory 6: Optical Sectioning Techniques (point scanning, line scanning, spinning disk), 2 x 2hr modules Lab Leaders: K Venkatesan Iyer and Mahipal Ganji Equipment 1: Zeiss LSM 980 Confocal Microscope Lab Instructor: Satyajit Mayor & Zeiss Application Specialist Lab Support: Piyush Chajjed Location: CIFF SLC
Equipment 2: Olympus FV3000 Confocal Microscope Lab Instructor: Arjun Guha & Olympus Application Specialist Lab Support: Nivya Mendon Location: CIFF inStem
Equipment 3: Olympus FV3000 Confocal Microscope Lab Instructor: Kedar Khare & Olympus Application Specialist Lab Support: Shivangi Pandey Location: CIFF SLC
Equipment 4: Olympus FV3000 Confocal Microscope Lab Instructor: Nicholas Carter & Olympus Application Specialist Lab Support: Raman S Location: CIFF SLC
Equipment 5: Olympus FV3000 Confocal Microscope Lab Instructor: Minhaj Sirajuddin & Olympus Application Specialist Lab Support: Heera Lal Location: CIFF inStem
Equipment 6: Nikon AXR Confocal Microscope Lab Instructor: Anjana Badarinarayanan & Nikon Application Specialist Lab Support: Inhere SA Location: CIFF SLC
Equipment 7: Olympus FV4000 Confocal Microscope Lab Instructor: Robert Cross and Olympus Application Specialist Lab Support: Drisya Dileep Location:
Equipment 8: Zeiss LSM 780 Confocal Microscope Lab Instructor: Lab Support: Location: CIFF SLC |
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12:45 – 13:30 |
Lunch Location: NCBS Canteen 1st Floor |
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15:30 – 16:30 |
Didactic Lecture 9: Super-resolution Microscopy – STED and SIM – Kedar Khare Location: Dasheri |
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16:30-17:00 |
Zeeshan Khan memorial Award Location: Dasheri |
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17:00 – 17:15 |
Coffee Break Location: Outside Dasheri |
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17:15-19:30 |
Laboratory 7: Image Processing / Analysis Workshop Lab leader: Robert Cross and Nicholas Carter Lab Support: Lakshmi Balasubramanian, Sukanta Behera, Swarang Pundlik, Heera Lal Location: Raspuri |
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19:30 – 20:15 |
Dinner Location: NCBS Canteen First floor |
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20:15 – 23:00 |
Cultural and Social Event Location: SLC Colonnade |
Thursday, Sept 26th: Specialized Techniques
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8:30-9:30 |
Didactic Lecture 10: Fluorescence Microscopy based methods to study protein dynamics in live cells: from FRAP to FCS – Thorsten Wohland Location: Haapus(LH1) |
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9:30-10:30 |
Didactic Lecture 11: Illuminating the Future: Advances and Horizons in Spatial Biology - Kevin Mathew Byrd Location: Online, Haapus(LH1)
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10:30 -10:45 |
Coffee Break Location: Outside Haapus(LH1) |
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10:45 – 14:45 |
Laboratory 8: Specialized techniques (2 x 1.5 hr modules)
Technique 1: Stimulated Emission Depletion Imaging Equipment: Abberior STED Microscope Lab Leader: Kedar Khare Lab Support: Gaurav Singh, Rayees Ganie, Pranjali Priya Location: CIFF SLC
Technique 2: STORM – DNA Paint Equipment: Nikon H-TIRF Lab Leader: Mahipal Ganji Lab Instructor: Rahul Roy Lab Support: Abhinav Bannerjee, Devyani Chakraborty, Inchara SA Location: Anjana Lab, SLC
Technique 3: Hetero FRET Imaging Equipment: Olympus FV3000 Confocal Microscope Lab Leader: Satyajit Mayor Lab Instructor: Olympus Application Specialist Lab Support: Swarang Pundlik, Sucheta Dey Location: CIFF inStem
Technique 4: Structured Illumination Microscopy Equipment: Zeiss Elyra Lattice SIM Lab Leader: Kedar Khare Lab Instructor: Zeiss Application Specialist Lab Support: Sukanta Behera Location: CIFF SLC
Technique 5: Super-resolution by Expansion Microscopy Equipment: Zeiss LSM 980 Confocal Microscope Lab Leader: Suliana Manley Lab Instructor: Swadhin Jana Lab Support: Pranjali Priya, Sucheta Dey Location: CIFF SLC
Technique 6: Total Internal Reflection Fluorescence (TIRF) Microscopy Equipment: Nikon H-TIRF Lab Leader: Minhaj Sirajuddin Lab Instructor: Rayees Ganie Lab Support: Sanjana Mullick Location: Minhaj Lab, inStem
Technique 7: Spatial Transcriptomics Equipment: 10x Genomics CytAssist and other equipment Lab Leader: Arjun Guha Lab Instructor: 10x Genomics Application specialist Lab Support: Neenu Joy, Vasam Manjeevkar Prabhantu Location: inStem
Technique 8: Protein Dynamics by FRAP and FCS Equipment: Zeiss LSM 780 Lab Leader: Thorsten Wohland Lab Instructor: Zeiss Application Specialist Lab Support: Sankarshan Talluri Location: CIFF SLC |
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12:15 – 13:15 |
Lunch Location: NCBS Canteen 1st floor |
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14:45- 15:00 |
Coffee Break Location: Outside Raspuri |
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15:00 – 16:00 |
Didactic Lecture 12: Optical Traps – Pramod Pullarkat Location: Haapus(LH1) |
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16:00 – 19:00 |
Non-Commercial Technical Presentation Location: Haapus(LH1) |
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19:00 – 20:00 |
Dinner Location: NCBS Canteen 1st floor |
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20:00 – 21:00 |
Online Demo of SMLM system by Abbelight Location: Raspuri |
Friday, Sept 27th: Research Lectures, Specialized Techniques
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8:30-9:15 |
Research Lecture 3: Robert Cross, University of Warwick Location: Dasheri |
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9:15-10:00 |
Research lecture 4: Suliana Manley, EPFL Location: Dasheri |
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10:00 -10:15 |
Coffee Break Location: Outside Dasheri |
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10:15 – 15:45 |
Laboratory 9: Specialized techniques (2 x 2hr modules)
Technique 1: Stimulated Emission Depletion Imaging Equipment: Abberior STED Microscope Lab Leader: Gaurav Singh Lab Support: Rayees Ganie, Pranjali Priya Location: CIFF SLC
Technique 2: STORM – DNA Paint Equipment: Nikon H-TIRF Lab Leader: Suliana Manley Lab Instructor: Mahipal Ganji & Rahul Roy Lab Support: Abhinav Bannerjee, Devyani Chakraborty Location: Anjana lab, SLC
Technique 3: Hetero-FRET Imaging Equipment: Olympus FV3000 Confocal Microscope Lab Leader: Satyajit Mayor Lab Instructor: Olympus Imaging Specialist Lab Support: Swarang Pundlik, Sucheta Dey Location: CIFF SLC
Technique 4: Structured Illumination Microscopy Equipment: Zeiss Elyra Lattice SIM Lab Leader: K Venkatesan Iyer Lab Instructor: Zeiss Application Specialist Lab Support: Sukanta Behera Location: CIFF SLC
Technique 5: Super-resolution by Expansion Microscopy Equipment: Zeiss LSM 980 Confocal Microscope Lab Leader: Suliana Manley Lab Instructor: Swadhin Jana Lab Support: Pranjali Priya, Sucheta Dey Location: CIFF SLC
Technique 6: Total Internal Reflection Fluorescence (TIRF) Microscopy Equipment: Nikon H-TIRF Lab Leader: Minhaj Sirajuddin Lab Instructor: Rayees Ganie Lab Support: Sanjana Mullick Location: Minhaj Lab, inStem
Technique 7: Spatial Transcriptomics Equipment: 10x Genomics CytAssist and other equipment Lab Leader: Arjun Guha Lab Instructor: 10x Genomics Application specialist Lab Support: Neenu Joy, Vasam Manjeevkar Prabhantu Location: inStem
Technique 8: Protein dynamics by FRAP and FCS Equipment: Zeiss LSM 780 Lab Leader: Thorsten Wohland Lab Instructor: Zeiss Application Specialist Lab Support: Sankarshan Talluri Location: CIFF SLC |
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12:15 – 12:30 |
Group Photo Location: Lawn outside Dasheri |
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12:30 – 13:45 |
Discussion over Lunch: Small group discussions of students with faculty over lunch about their research/ microscopy interests Location: NCBS Canteen 1st floor |
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15:45 – 16:00 |
Coffee Break Location: Outside Dasheri |
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16:00-17:00 |
Didactic Lecture 13: Lightsheet Imaging – Thorsten Wohland Location: Dasheri |
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17:00-17:45 |
Research Lecture 5: Mahipal Ganji Location: Dasheri |
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17:45 – 18:30 |
Research Lecture 6: Thorsten Wohland, National University of Singapore Location: Dasheri |
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18:30 – 19:30 |
Graduation Location: Dasheri |
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19:30 – 23:00 |
Workshop Dinner - Downtown |
Saturday, Sept 28th: Projects
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9:00-9:45 |
Research Lecture 7: Arjun Guha, inStem Location: Dasheri |
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9:45-10:30 |
Research lecture 8: Vinothkumar Kutti Raghunath, NCBS, Bangalore, India Location: Dasheri |
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10:30 -10:45 |
Coffee Break Location: Outside Dasheri |
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10:45 – 11:00 |
Project briefing |
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11:00 – 13:30 |
Projects |
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13:30 – 14:30 |
Lunch Location: NCBS Canteen 1st Floor |
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14:30 – 16:30 |
Projects |
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16:30-16:45 |
Coffee Break Location: Outside Raspuri |
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16:45 – 19:30 |
Projects |
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19:30 – 20: 30 |
Dinner Location: NCBS Canteen First floor |
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20:30 – 23:00 |
Projects / Free Time on Microscopes |
Sunday, Sept 29th: Projects
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9:00-11:00 |
Projects |
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11:00-11:15 |
Coffee Break Location: Outside Raspuri |
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11:15 – 13:00 |
Projects |
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13:00 – 14:00 |
Lunch Location: NCBS Canteen 1st Floor |
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14:00 – 16:00 |
Projects |
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16:00 – 16:15 |
Coffee Break Location: Outside Raspuri |
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16:15 – 18:15 |
Project presentation by participants Location: LH1 |
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18:15 – 19:00 |
Closing Remarks Location: LH1 |
