
@article {152149,
	title = {Interplay of geometry and mechanics in epithelial wound healing.},
	journal = {Phys Rev E},
	volume = {110},
	year = {2024},
	month = {2024 Nov},
	pages = {054411},
	abstract = {<p>Wound healing is a complex biological process critical for maintaining an organism{\textquoteright}s structural integrity and tissue repair following an infection or injury. Recent studies have unveiled the mechanisms involving the coordination of biochemical and mechanical responses in the tissue in wound healing. In this article, we focus on the healing property of an epithelial tissue as a material while the effects of biological mechanisms such as cell proliferation, tissue intercalation, cellular migration, cell crawling, and filopodia protrusion is minimal. We present a mathematical framework that predicts the fate of a wounded tissue based on the wound{\textquoteright}s geometrical features and the tissue{\textquoteright}s mechanical properties. Precisely, adapting the vertex model of tissue mechanics, we predict whether a wound of a specific size in an epithelial monolayer characterized by certain levels of actomyosin contractility and cell-cell adhesion will heal (i.e., close), shrink in size, or rupture the tissue further. Moreover, we show how tissue-mediated mechanisms such as purse-string tension at the wound boundary facilitate wound healing. Finally, we validate the predictions of our model by designing an experimental setup that enables us to create wounds of specific sizes in kidney epithelial cells (MDCK) monolayers. Altogether, this work sets up a basis for interpreting the interplay of mechanical and geometrical features of a tissue in the process of wound healing.</p>
},
	keywords = {Animals, Biomechanical Phenomena, Dogs, Epithelial Cells, Epithelium, Madin Darby Canine Kidney Cells, Mechanical Phenomena, Models, Biological, Wound Healing},
	issn = {2470-0053},
	doi = {10.1103/PhysRevE.110.054411},
	author = {Babu, Nandhu Krishna and Sreepadmanabh, M and Dutta, Sayantan and Bhattacharjee, Tapomoy}
}
@article {117962,
	title = {Initiation of wound healing is regulated by the convergence of mechanical and epigenetic cues.},
	journal = {PLoS Biol},
	volume = {20},
	year = {2022},
	month = {2022 Sep},
	pages = {e3001777},
	abstract = {<p>Wound healing in the skin is a complex physiological process that is a product of a cell state transition from homeostasis to repair. Mechanical cues are increasingly being recognized as important regulators of cellular reprogramming, but the mechanism by which it is translated to changes in gene expression and ultimately cellular behavior remains largely a mystery. To probe the molecular underpinnings of this phenomenon further, we used the down-regulation of caspase-8 as a biomarker of a cell entering the wound healing program. We found that the wound-induced release of tension within the epidermis leads to the alteration of gene expression via the nuclear translocation of the DNA methyltransferase 3A (DNMT3a). This enzyme then methylates promoters of genes that are known to be down-regulated in response to wound stimuli as well as potentially novel players in the repair program. Overall, these findings illuminate the convergence of mechanical and epigenetic signaling modules that are important regulators of the transcriptome landscape required to initiate the tissue repair process in the differentiated layers of the epidermis.</p>
},
	keywords = {Biomarkers, Caspase 8, Cues, Epigenesis, Genetic, Wound Healing},
	issn = {1545-7885},
	doi = {10.1371/journal.pbio.3001777},
	author = {Bhatt, Tanay and Dey, Rakesh and Hegde, Akshay and Ketkar, Alhad Ashok and Pulianmackal, Ajai J and Deb, Ashim P and Rampalli, Shravanti and Jamora, Colin}
}
