
@article {86473,
	title = {Impact of Temperature and Time Interval Prior to Immature Testicular-Tissue Organotypic Culture on Cellular Niche.},
	journal = {Reprod Sci},
	year = {2020},
	month = {2020 Dec 15},
	abstract = {<p>Cryopreservation of immature-testicular-tissue (ITT) prior to gonadotoxic treatment, while experimental, is the only recommended option for fertility preservation in prepubertal boys. The handling and manipulation of ITT before cryopreservation could influence the functionality of cells during fertility restoration, which this study explored by evaluating cellular niche and quality of mouse ITT subjected to various temperatures and time durations in vitro. ITT from 6-day-old mice were handled at ultraprofound-hypothermic, profound-hypothermic, and mild-warm-ischemic temperatures for varying time periods prior to 14-day organotypic culture. Viability, functionality, synaptonemal complex and chromatin remodeling markers were assessed. Results have shown that cell viability, testosterone level, and in vitro proliferation ability did not change when ITT were held at ultraprofound-hypothermic-temperature up to 24\&nbsp;h, whereas cell viability was significantly reduced (P \&lt; 0.01), when held at profound-hypothermic-temperature for 24\&nbsp;h before culture. Further, cell viability and testosterone levels in cultured cells from profound-hypothermic group were comparable to corresponding ultraprofound-hypothermic group but with moderate reduction in postmeiotic cells (P \&lt; 0.01). In conclusion, holding ITT at ultraprofound-hypothermic-temperature is most suitable for organotypic culture, whereas short-term exposure at profound-hypothermic-temperature may compromise postmeiotic germ cell yield post in vitro culture. This data, albeit in mouse model, will have immense value in human prepubertal fertility restoration research.</p>
},
	issn = {1933-7205},
	doi = {10.1007/s43032-020-00396-z},
	author = {Salian, Sujith Raj and Pandya, Riddhi Kirit and Laxminarayana, Sindhura Lakshmi Koulmane and Krishnamurthy, Hanumantappa and Cheredath, Aswathi and Tholeti, Prathima and Uppangala, Shubhashree and Kalthur, Guruprasad and Majumdar, Subeer and Schlatt, Stefan and Adiga, Satish Kumar}
}
@article {11602,
	title = {Unraveling the association between genetic integrity and metabolic activity in pre-implantation stage embryos.},
	journal = {Sci Rep},
	volume = {6},
	year = {2016},
	month = {2016 Nov 17},
	pages = {37291},
	abstract = {<p>Early development of certain mammalian embryos is protected by complex checkpoint systems to maintain the genomic integrity. Several metabolic pathways are modulated in response to genetic insults in mammalian cells. The present study investigated the relationship between the genetic integrity, embryo metabolites and developmental competence in preimplantation stage mouse embryos with the aim to identify early biomarkers which can predict embryonic genetic integrity using spent medium profiling by NMR spectroscopy. Embryos carrying induced DNA lesions (IDL) developed normally for the first 2.5 days, but began to exhibit a developmental delay at embryonic day 3.5(E3.5) though they were morphologically indistinguishable from control embryos. Analysis of metabolites in the spent medium on E3.5 revealed a significant association between pyruvate, lactate, glucose, proline, lysine, alanine, valine, isoleucine and thymine and the extent of genetic instability observed in the embryos on E4.5. Further analysis revealed an association of apoptosis and micronuclei frequency with P53 and Bax transcripts in IDL embryos on the E4.5 owing to delayed induction of chromosome instability. We conclude that estimation of metabolites on E3.5 in spent medium may serve as a biomarker to predict the genetic integrity in pre-implantation stage embryos which opens up new avenues to improve outcomes in clinical IVF programs.</p>
},
	issn = {2045-2322},
	doi = {10.1038/srep37291},
	author = {D{\textquoteright}Souza, Fiona and Pudakalakatti, Shivanand M and Uppangala, Shubhashree and Honguntikar, Sachin and Salian, Sujith Raj and Kalthur, Guruprasad and Pasricha, Renu and Appajigowda, Divya and Atreya, Hanudatta S and Adiga, Satish Kumar}
}
