
@article {73645,
	title = {A coherent feed forward loop drives vascular regeneration in damaged aerial organs growing in normal developmental-context.},
	journal = {Development},
	year = {2020},
	month = {2020 Feb 27},
	abstract = {<p>Aerial organs of plants being highly prone to local injuries, require tissue restoration to ensure their survival. However, knowledge of the underlying mechanism is sparse. In this study, we mimicked natural injuries in growing leaf and stem to study the reunion between mechanically disconnected tissues. We show that ()/ () genes, which encodes stem cell promoting factors, are activated and contribute to vascular regeneration in response to these injuries. PLT proteins bind to and activate the CUC2 promoter. Both PLT and CUC2 regulate the transcription of the local auxin biosynthesis gene YUC4 in a coherent feed forward loop, and this process is necessary to drive vascular regeneration. In the absence of this PLT mediated regeneration response, leaf ground tissue cells can neither acquire early vascular identity marker ATHB8, nor properly polarize auxin transporters to specify new venation paths. The PLT-CUC2 module is required for vascular regeneration, but is dispensable for midvein formation in leaf. We reveal the mechanisms of vascular regeneration in plants and distinguishes the wound repair ability of the tissue from its formation during normal development.</p>
},
	issn = {1477-9129},
	doi = {10.1242/dev.185710},
	author = {Radhakrishnan, Dhanya and Shanmukhan, Anju Pallipurath and Kareem, Abdul and Aiyaz, Mohammed and Varapparambathu, Vijina and Toms, Ashna and Kerstens, Merijn and Valsakumar, Devisree and Landge, Amit N and Shaji, Anil and Mathew, M K and Sawchuk, Megan G and Scarpella, Enrico and Krizek, Beth A and Efroni, Idan and M{\"a}h{\"o}nen, Ari Pekka and Willemsen, Viola and Scheres, Ben and Prasad, Kalika}
}
@article {57787,
	title = {The Ca2+ Channel CNGC19 Regulates Arabidopsis defense against Spodoptera Herbivory.},
	journal = {Plant Cell},
	year = {2019},
	month = {2019 May 10},
	abstract = {<p>Cellular calcium elevation is an important signal used by plants for recognition and signaling of environmental stress. Perception of the generalist insect, Spodoptera litura, by Arabidopsis thaliana activates cytosolic Ca2+ elevation, which triggers downstream defense. However, not all the Ca2+ channels generating the signal have been identified, nor are their modes of action known. We report on a rapidly activated, leaf vasculature- and plasma membrane-localized, CYCLIC NUCLEOTIDE GATED CHANNEL19 (CNGC19), which activates herbivory-induced Ca2+ flux and plant defense. Loss of CNGC19 function results in decreased herbivory defense. The cngc19 mutant shows aberrant and attenuated intra-vascular Ca2+ fluxes. CNGC19 is a Ca2+ permeable channel, as hyperpolarization of CNGC19-expressing Xenopus oocytes in the presence of both cAMP and Ca2+ results in Ca2+ influx. Breakdown of Ca2+-based defence in cngc19 mutants leads to a decrease in herbivory-induced JA-Ile biosynthesis and expression of JA responsive genes. cngc19 mutants are deficient in aliphatic glucosinolate accumulation and hyperaccumulate its precursor, methionine. CNGC19 modulates aliphatic glucosinolate biosynthesis in tandem with BRANCHED-CHAIN AMINO ACID TRANSAMINASE4 (BCAT4), which is involved in the chain elongation pathway of Met-derived glucosinolates. Furthermore, CNGC19 interacts with herbivory-induced CALMODULIN2 (CaM2) in planta. Together, our work reveals a key mechanistic role for the Ca2+ channel CNGC19 in the recognition of herbivory and the activation of defense signaling.</p>
},
	issn = {1532-298X},
	doi = {10.1105/tpc.19.00057},
	author = {Meena, Mukesh Kumar and Prajapati, Ramgopal and Krishna, Deepthi and Divakaran, Keerthi and Pandey, Yogesh and Reichelt, Michael and Mathew, M K and Boland, Wilhelm and Mith{\"o}fer, Axel and Vadassery, Jyothilakshmi}
}
