
@article {152904,
	title = {Is Drosophila Larval Competition Involved in Incipient Speciation?},
	journal = {J Chem Ecol},
	volume = {51},
	year = {2025},
	month = {2025 Jan 22},
	pages = {2},
	abstract = {<p>Geographical, ethological, temporal and ecological barriers can affect interbreeding between populations deriving from an ancestral population, this progressively leading to speciation. A rare case of incipient speciation currently occurs between Drosophila melanogaster populations sampled in Zimbabwe (Z) and all other populations (M). This phenomenon was initially characterized by Z females refusing to mate with M males. Despite the fact that Z and M flies produce different amounts of cuticular pheromones, their manipulation and that of other sensory signals exchanged during courtship behavior only marginally rescued the behavioral isolation. To further explore the putative mechanisms involved in this phenomenon, we first assessed the fecundity in matings between Z and M flies. Then, we measured the reproduction and survival in adults resulting of co-cultured Z and M larvae. In these two experiments, Z flies rarely emerged. Z and M larvae produced different amounts of food-derived metabolites which were altered in co-culture condition. This maybe related to the different bacteria composition in the gut and body of Z and M flies. However, the mating behavior of co-cultured flies did not change and their cuticular pheromone profile was slightly altered. Thus, the Z/M larval competition could reinforce the barriers induced by gametic and behavioral isolation processes on this incipient speciation phenomenon.</p>
},
	keywords = {Animals, Competitive Behavior, Drosophila melanogaster, Female, Fertility, Genetic Speciation, Larva, Male, Pheromones, Reproduction, Sexual Behavior, Animal},
	issn = {1573-1561},
	doi = {10.1007/s10886-025-01560-y},
	author = {Jean-Fran{\c c}ois, Ferveur and Pratibha, Sanjenbam and Baptiste, Regnier and Jean-Pierre, Farine and J{\'e}r{\^o}me, Cortot and Deepa, Agashe and Claude, Everaerts}
}
@article {150946,
	title = {Experimental Evolution Induced by Maternal Post-copulatory Factors in Drosophila.},
	journal = {Behav Genet},
	year = {2024},
	month = {2024 Nov 21},
	abstract = {<p>Experimental evolution is a powerful approach to study the mechanisms underlying the adaptation of selected characters under the conditions chosen in the laboratory. Drosophila melanogaster is a species frequently used to investigate the experimental evolution of characters, especially those related to reproduction. Recent intra-generational studies showed that cis-vaccenyl acetate (cVa), a sex pheromone transferred with bacteria on eggs by females either 1\&nbsp;day (D1) or 5\&nbsp;days (D5) after copulation, differentially affected the behavior and pheromone release in adult males emerging from these eggs. Here, we extended this finding to determine whether this alternative egg exposure repeated over many generations could affect a larger set of reproduction-related characters in both sexes. To test the repetitive effects of maternal D1 or D5 post-copulatory factors, we carried out an experimental selection procedure consisting of exposing eggs during 40 successive generations to D1 or D5 maternal post-copulatory factors. We compared cVa and cuticular pheromones, courtship and mating behaviors, and fecundity at different generations in flies of D1 and D5 lines. Based on findings obtained at earlier generations, we also determined survival, bacterial composition and gene expression in adults. Some of these complex traits significantly diverged between D1 and D5 lines indicating that maternal post-copulatory factors transmitted to eggs can influence adult life history traits.</p>
},
	issn = {1573-3297},
	doi = {10.1007/s10519-024-10206-w},
	author = {Jean-Fran{\c c}ois, Ferveur and Pratibha, Sanjenbam and St{\'e}phane, Fraichard and Enisa, Aru{\c c}i and Fabrice, Neiers and Bernard, Moussian and Deepa, Agashe and Claude, Everaerts}
}
