
@article {140648,
	title = {IFT88 maintains sensory function by localising signalling proteins along  cilia.},
	journal = {Life Sci Alliance},
	volume = {7},
	year = {2024},
	month = {2024 May},
	abstract = {<p>Ciliary defects cause several ciliopathies, some of which have late onset, suggesting cilia are actively maintained. Still, we have a poor understanding of the mechanisms underlying their maintenance. Here, we show r IFT88 (IFT88/nompB) continues to move along fully formed sensory cilia. We further identify Inactive, a TRPV channel subunit involved in hearing and negative-gravitaxis behaviour, and a yet uncharacterised Guanylyl Cyclase 2d (Gucy2d/CG34357) as IFT88 cargoes. We also show IFT88 binding to the cyclase{\textasciiacute}s intracellular part, which is evolutionarily conserved and mutated in several degenerative retinal diseases, is important for the ciliary localisation of Gucy2d. Finally, acute knockdown of both IFT88 and Gucy2d in ciliated neurons of adult flies caused defects in the maintenance of cilium function, impairing hearing and negative-gravitaxis behaviour, but did not significantly affect ciliary ultrastructure. We conclude that the sensory ciliary function underlying hearing in the adult fly requires an active maintenance program which involves IFT88 and at least two of its signalling transmembrane cargoes, Gucy2d and Inactive.</p>
},
	keywords = {Animals, Cilia, Drosophila, Drosophila melanogaster, Drosophila Proteins, Hearing},
	issn = {2575-1077},
	doi = {10.26508/lsa.202302289},
	author = {Werner, Sascha and Okenve-Ramos, Pilar and Hehlert, Philip and Zitouni, Sihem and Priya, Pranjali and Mendon{\c c}a, Susana and Sporbert, Anje and Spalthoff, Christian and G{\"o}pfert, Martin C and Jana, Swadhin Chandra and Bettencourt-Dias, M{\'o}nica}
}
