
@article {148051,
	title = {Molecular Dialogue During Host Manipulation by the Vascular Wilt Fungus .},
	journal = {Annu Rev Phytopathol},
	volume = {62},
	year = {2024},
	month = {2024 Sep},
	pages = {97-126},
	abstract = {<p>Vascular wilt fungi are a group of hemibiotrophic phytopathogens that infect diverse crop plants. These pathogens have adapted to thrive in the nutrient-deprived niche of the plant xylem. Identification and functional characterization of effectors and their role in the establishment of compatibility across multiple hosts, suppression of plant defense, host reprogramming, and interaction with surrounding microbes have been studied mainly in model vascular wilt pathogens and . Comparative analysis of genomes from fungal isolates has accelerated our understanding of genome compartmentalization and its role in effector evolution. Also, advances in recent years have shed light on the cross talk of root-infecting fungi across multiple scales from the cellular to the ecosystem level, covering their interaction with the plant microbiome as well as their interkingdom signaling. This review elaborates on our current understanding of the cross talk between vascular wilt fungi and the host plant, which eventually leads to a specialized lifestyle in the xylem. We particularly focus on recent findings in , including multihost associations, and how they have contributed to understanding the biology of fungal adaptation to the xylem. In addition, we discuss emerging research areas and highlight open questions and future challenges.</p>
},
	keywords = {Fusarium, Host-Pathogen Interactions, Plant Diseases, Xylem},
	issn = {1545-2107},
	doi = {10.1146/annurev-phyto-021722-034823},
	author = {Srivastava, Vidha and Patra, Kuntal and Pai, Hsuan and Aguilar-Pontes, Maria Victoria and Berasategui, Aileen and Kamble, Avinash and Di Pietro, Antonio and Redkar, Amey}
}
@article {129166,
	title = {Live-Cell Visualization of Early Stages of Root Colonization by the Vascular Wilt Pathogen Fusarium oxysporum.},
	journal = {Methods Mol Biol},
	volume = {2659},
	year = {2023},
	month = {2023},
	pages = {73-82},
	abstract = {<p>Fungal phytopathogens induce a variety of pathogenicity symptoms on their hosts. The soilborne vascular wilt pathogen Fusarium oxysporum infects roots of more than 150 different crop species. Initial colonization stages are asymptomatic, likely representing a biotrophic phase of infection, followed by a necrotrophic switch after vascular colonization which results in blockage of the plant xylem and killing of the host. Live-cell microscopy techniques have been successfully employed to study interaction events during fungal colonization of root tissues. This technique is widely used to track fungal development during disease progression. Here, we describe a well-established protocol for generation and screening of fluorescently tagged F. oxysporum transformants, as well as for live-cell imaging of the early colonization stages of F. oxysporum on tomato (Solanum lycopersicum) seedlings. The presented experimental design and techniques involved are also applicable to other root infecting fungi.</p>
},
	keywords = {Fusarium, Plant Diseases, Solanum lycopersicum, Virulence},
	issn = {1940-6029},
	doi = {10.1007/978-1-0716-3159-1_6},
	author = {Redkar, Amey and Di Pietro, Antonio and Turr{\`a}, David}
}
