
@article {128075,
	title = {Optimized protocol for assay for transposase-accessible chromatin by sequencing (ATAC-seq) from Drosophila melanogaster brain tissue.},
	journal = {STAR Protoc},
	volume = {4},
	year = {2023},
	month = {2023 Apr 18},
	pages = {102153},
	abstract = {<p>Transposase-accessible chromatin by sequencing (ATAC-seq) has emerged as an advantageous technique to assess chromatin accessibility owing to the robustness of "tagmentation" process and a relatively faster library preparation. A\&nbsp;comprehensive ATAC-seq protocol from Drosophila brain tissue is currently unavailable. Here, we have provided a detailed protocol of ATAC-seq assay from Drosophila brain tissue. Starting from dissection and transposition to amplification of libraries has been elaborated. Furthermore, a robust ATAC-seq analysis pipeline has been presented. The protocol can be easily adapted for other soft tissues.</p>
},
	issn = {2666-1667},
	doi = {10.1016/j.xpro.2023.102153},
	author = {Dhall, Jasmine Kaur and Kasturacharya, Nandashree and Pandit, Awadhesh and Cp, Lakshminarayanan}
}
