@article {121149, title = {Molecular markers for early stratification of disease severity and progression in COVID-19.}, journal = {Biol Methods Protoc}, volume = {7}, year = {2022}, month = {2022}, pages = {bpac028}, abstract = {

COVID-19 infections have imposed immense pressure on the healthcare system of most countries. While the initial studies have identified better therapeutic and diagnostic approaches, the disease severity is still assessed by close monitoring of symptoms by healthcare professionals due to the lack of biomarkers for disease stratification. In this study, we have probed the immune and molecular profiles of COVID-19 patients at 48-h intervals after hospitalization to identify early markers, if any, of disease progression and severity. Our study reveals that the molecular profiles of patients likely to enter the host-immune response-mediated moderate or severe disease progression are distinct even in the early phase of infection when severe symptoms are not yet apparent. Our data from 37 patients suggest that at hospitalization, interleukins (IL6) (\>300\ pg/ml) and IL8 levels (\>200\ pg/ml) identify cytokine-dependent disease progression. Monitoring their levels will facilitate timely intervention using available immunomodulators or precision medicines in those likely to progress due to cytokine storm and help improve outcomes. Additionally, it will also help identify cytokine-independent progressive patients, not likely to benefit from immunomodulators or precision drugs.

}, issn = {2396-8923}, doi = {10.1093/biomethods/bpac028}, author = {Kashyap, Anusha and Sebastian, Savitha Anne and Krishnaiyer NarayanaSwamy, Sree Raksha and Raksha, KalyanKumar and Krishnamurthy, Hanumanthappa and Krishna, Bhuvana and D{\textquoteright}Souza, George and Idiculla, Jyothi and Vyas, Neha} } @article {83644, title = {Induction of Redox-Mediated Cell Death in ER-Positive and ER-Negative Breast Cancer Cells by a Copper(II)-Phenolate Complex: An In Vitro and In Silico Study.}, journal = {Molecules}, volume = {25}, year = {2020}, month = {2020 Oct 01}, abstract = {

This research was aimed at finding the cytotoxic potential of the mixed ligand copper(II) complex [Cu(tdp)(phen)](ClO)-where H(tdp) is the tetradentate ligand 2-[(2-(2-hydroxyethylamino)-ethylimino)methyl]phenol, and phen is 1,10-phenanthroline-to two genotypically different breast cancer cells, MCF-7 (p53 and ER) and MDA-MB-231 (p53 and ER). The complex has been already shown to be cytotoxic to ME180 cervical carcinoma cells. The special focus in this study was the induction of cell death by apoptosis and necrosis, and its link with ROS. The treatment brought about nuclear fragmentation, phosphatidylserine externalization, disruption of mitochondrial trans-membrane potential, DNA damage, cell cycle arrest at sub-G1 phase, and increase of ROS generation, followed by apoptotic death of cells during early hours and a late onset of necrosis in the cells surviving the apoptosis. The efficacy of the complex against genotypically different breast cancer cells is attributed to a strong association through p53-mitochondrial redox-cell cycle junction. The ADMET properties and docking of the complex at the active site of Top1 are desirable attributes of a lead molecule for development into a therapeutic. Thus, it is shown that the copper(II)-phenolate complex[Cu(tdp)(phen)] offers potential to be developed into a therapeutic for breast cancers in general and ER-negative ones in particular.

}, issn = {1420-3049}, doi = {10.3390/molecules25194504}, author = {Periasamy, Vaiyapuri Subbarayan and Riyasdeen, Anvarbatcha and Rajendiran, Venugopal and Palaniandavar, Mallayan and Krishnamurthy, Hanumanthappa and Alshatwi, Ali Abdullah and Akbarsha, Mohammad Abdulkader} } @article {3565, title = {Ethanolic extract of Moringa oleifera Lam. leaves protect the pre-pubertal spermatogonial cells from cyclophosphamide-induced damage.}, journal = {J Ethnopharmacol}, volume = {182}, year = {2016}, month = {2016 Apr 22}, pages = {101-9}, abstract = {

ETHNOPHARMACOLOGICAL RELEVANCE: Moringa oleifera Lam. is widely cultivated in Asian and African countries for its medicinal and dietary significance. The leaves are highly nutritious and are known to possess various biological activities.

MATERIALS AND METHODS: Pre-pubertal Swiss albino male mice were injected with single dose of cyclophosphamide (CP, 200mg/kg body weight) or ethanolic extract of Moringa oleifera leaves (MOE, 100mg/kg body weight) intraperitoneally. In combination group, MOE was administered 24h prior to CP injection.

RESULTS: CP induced a significant decrease in testicular weight (p\<0.01) and depletion of germ cells (p\<0.001) and higher level of DNA damage (p\<0.001) compared to control. The expression of P53, Bax, Cytochrome C (Cyt C) was increased while there was a decrease in the expression of Bcl2, c-Kit and Oct4. Administration of MOE 24h prior to CP treatment ameliorated the depletion (p\<0.001), DNA damage (p\<0.001) and apoptosis (p\<0.01) of germ cells induced by CP. The mitigating effect of MOE appears to be mediated by up-regulating the expression of c-Kit and Oct4 transcripts in P53-independent manner.

CONCLUSION: MOE protects the spermatogonial cells from CP-induced damage by modulating the apoptotic response elicited by CP and therefore can be considered as an efficient method of male fertility preservation.

}, issn = {1872-7573}, doi = {10.1016/j.jep.2016.02.003}, author = {Nayak, Guruprasad and Honguntikar, Sachin D and Kalthur, Sneha Guruprasad and D{\textquoteright}Souza, Antony Sylvan and Mutalik, Srinivas and Setty, Manjunath M and Kalyankumar, Raksha and Krishnamurthy, Hanumanthappa and Kalthur, Guruprasad and Adiga, Satish Kumar} }