
@article {109309,
	title = {Development of candidate gene-based markers and map-based cloning of a dominant rust resistance gene in cultivated groundnut (Arachis hypogaea L.).},
	journal = {Gene},
	volume = {827},
	year = {2022},
	month = {2022 Apr 04},
	pages = {146474},
	abstract = {<p>A dominant rust resistance gene, VG 9514-Rgene was isolated through map-based cloning. Sequence analysis revealed non-synonymous mutations in the TIR, NBS and LRR region of the R-protein. Candidate gene-based markers from these SNPs revealed complete co-segregation of the isolated VG 9514-Rgene with rust resistance in a RIL population and confirmed their map position in between FRS 72 and SSR_GO340445 markers in arahy03 chromosome. Blastp search of VG 9514-Rprotein detected Arahy.T6DCA5 with \&gt;80.0\% identity that localized at 142,544,745.0.142,549,184 in arahy03 chromosome. K/K calculation revealed that VG 9514-Rgene had undergone positive selection compared to four homologous genes in the groundnut genome. Homology based structure modelling of this R-protein revealed a typical consensus three-dimensional folding of TIR-NBS-LRR protein. Non-synonymous mutations in susceptible version of R-protein were mapped and found E268Q mutation in hhGRExE motif, Y309F in RNBS-A motif and I579T in MHD motif of NB-ARC domain are probable candidates for loss of function.</p>
},
	issn = {1879-0038},
	doi = {10.1016/j.gene.2022.146474},
	author = {Mondal, Suvendu and Mohamed Shafi, K and Raizada, Avi and Song, Hui and Badigannavar, Anand M and Sowdhamini, Ramanathan}
}
