
@article {105161,
	title = {The Transcriptional Response to Oxidative Stress is Independent of Stress-Granule Formation.},
	journal = {Mol Biol Cell},
	year = {2022},
	month = {2022 Jan 05},
	pages = {mbcE21080418},
	abstract = {<p>Cells respond to stress with translational arrest, robust transcriptional changes, and transcription-independent formation of mRNP assemblies termed stress granules (SGs). Despite considerable interest in the role of SGs in oxidative, unfolded-protein and viral stress responses, whether and how SGs contribute to stress-induced transcription has not been rigorously examined. To address this, we characterized transcriptional changes in S2 cells induced by acute oxidative-stress and assessed how these were altered under conditions that disrupted SG assembly. Oxidative stress for 3-hours predominantly resulted in induction or upregulation of stress-responsive mRNAs whose levels peaked during recovery after stress cessation. The stress-transcriptome is enriched in mRNAs coding for chaperones, including HSP70s, small heat shock proteins, glutathione transferases, and several non-coding RNAs. Oxidative stress also induced cytoplasmic SGs that disassembled 3-hours after stress cessation. As expected, RNAi-mediated knockdown of the conserved G3BP1/Rasputin protein inhibited SG assembly. However, this disruption had no significant effect on the stress-induced transcriptional response or stress-induced translational arrest. Thus, SG assembly and stress-induced gene expression alterations appear to be driven by distinctive signaling processes. We suggest that while SG assembly represents a fast, transient mechanism, the transcriptional response enables a slower, longer-lasting mechanism for adaptation to and recovery from cell stress.</p>
},
	issn = {1939-4586},
	doi = {10.1091/mbc.E21-08-0418},
	author = {Singh, Amanjot and Kandi, Arvind Reddy and Jayaprakashappa, Deepa and Thuery, Guillaume and Purohit, Devam J and Huelsmeier, Joern and Singh, Rashi and Pothapragada, Sai Shruti and Ramaswami, Mani and Bakthavachalu, Baskar}
}
