
@article {100626,
	title = {Cargo-specific recruitment in clathrin- and dynamin-independent endocytosis.},
	journal = {Nat Cell Biol},
	year = {2021},
	month = {2021 Oct 06},
	abstract = {<p>Spatially controlled, cargo-specific endocytosis is essential for development, tissue homeostasis and cancer invasion. Unlike cargo-specific clathrin-mediated endocytosis, the clathrin- and dynamin-independent endocytic pathway (CLIC-GEEC, CG pathway) is considered a bulk internalization route for the fluid phase, glycosylated membrane proteins and lipids. While the core molecular players of CG-endocytosis have been recently defined, evidence of cargo-specific adaptors or selective uptake of proteins for the pathway are lacking. Here we identify the actin-binding protein Swiprosin-1 (Swip1, EFHD2) as a cargo-specific adaptor for CG-endocytosis. Swip1 couples active Rab21-associated integrins with key components of the CG-endocytic machinery-Arf1, IRSp53 and actin-and is critical for integrin endocytosis. Through this function, Swip1 supports integrin-dependent cancer-cell migration and invasion, and is a negative prognostic marker in breast cancer. Our results demonstrate a previously unknown cargo selectivity for the CG pathway and a role for specific adaptors in recruitment into this endocytic route.</p>
},
	issn = {1476-4679},
	doi = {10.1038/s41556-021-00767-x},
	author = {Moreno-Layseca, Paulina and J{\"a}ntti, Niklas Z and Godbole, Rashmi and Sommer, Christian and Jacquemet, Guillaume and Al-Akhrass, Hussein and Conway, James R W and Kronqvist, Pauliina and Kallionp{\"a}{\"a}, Roosa E and Oliveira-Ferrer, Leticia and Cervero, Pasquale and Linder, Stefan and Aepfelbacher, Martin and Zauber, Henrik and Rae, James and Parton, Robert G and Disanza, Andrea and Scita, Giorgio and Mayor, Satyajit and Selbach, Matthias and Veltel, Stefan and Ivaska, Johanna}
}
