
@article {77384,
	title = {Myosin II Filament Dynamics in Actin Networks Revealed with Interferometric Scattering Microscopy.},
	journal = {Biophys J},
	volume = {118},
	year = {2020},
	month = {2020 Apr 21},
	pages = {1946-1957},
	abstract = {<p>The plasma membrane and the underlying cytoskeletal cortex constitute active platforms for a variety of cellular processes. Recent work has shown that the remodeling acto-myosin network modifies local membrane organization, but the molecular details are only partly understood because of difficulties with experimentally accessing the relevant time and length scales. Here, we use interferometric scattering microscopy to investigate a minimal acto-myosin network linked to a supported lipid bilayer membrane. Using the magnitude of the interferometric contrast, which is proportional to molecular mass, and fast acquisition rates, we detect and image individual membrane-attached actin filaments diffusing within the acto-myosin network and follow individual myosin II filament dynamics. We quantify myosin II filament dwell times and processivity as functions of ATP concentration, providing experimental evidence for the predicted ensemble behavior of myosin head domains. Our results show how decreasing ATP concentrations lead to both increasing dwell times of individual myosin II filaments and a global change from a remodeling to a contractile state of the acto-myosin network.</p>
},
	issn = {1542-0086},
	doi = {10.1016/j.bpj.2020.02.025},
	author = {Mosby, Lewis S and Hundt, Nikolas and Young, Gavin and Fineberg, Adam and Polin, Marco and Mayor, Satyajit and Kukura, Philipp and K{\"o}ster, Darius V}
}
@article {3544,
	title = {Cortical actin and the plasma membrane: inextricably intertwined.},
	journal = {Curr Opin Cell Biol},
	volume = {38},
	year = {2016},
	month = {2016 Feb},
	pages = {81-9},
	abstract = {<p>The plasma membrane serves as a barrier, separating the cell from its external environment. Simultaneously it acts as a site for information transduction, entry of nutrients, receptor signaling, and adapts to the shape of the cell. This requires local control of organization at multiple scales in this heterogeneous fluid lipid bilayer with a plethora of proteins and a closely juxtaposed dynamic cortical cytoskeleton. New membrane models highlight the influence of the underlying cortical actin on the diffusion of membrane components. Myosin motors as well as proteins that remodel actin filaments have additionally been implicated in defining the organization of many membrane constituents. Here we provide a perspective of the intimate relationship of the membrane lipid matrix and the underlying cytoskeleton.</p>
},
	issn = {1879-0410},
	doi = {10.1016/j.ceb.2016.02.021},
	author = {K{\"o}ster, Darius V and Mayor, Satyajit}
}
