
@article {136528,
	title = {Cysteine-free cone snail venom peptides: Classification of precursor proteins and identification of mature peptides.},
	journal = {J Pept Sci},
	year = {2023},
	month = {2023 Nov 27},
	pages = {e3554},
	abstract = {<p>The cysteine-free acyclic peptides present in marine cone snail venom have been much less investigated than their disulfide bonded counterparts. Precursor protein sequences derived from transcriptomic data, together with mass spectrometric fragmentation patterns for peptides present in venom duct tissue extracts, permit the identification of mature peptides. Twelve distinct gene superfamiles have been identified with precursor lengths between 64 and 158 residues. In the case of Conus monile, three distinct mature peptides have been identified, arising from two distinct protein precursors. Mature acyclic peptides are often post-translationally modified, with C-terminus amidation, a feature characteristic of neuropeptides. In the present study, 20 acyclic peptides from Conus monile and Conus betulinus were identified. The common modifications of C-terminus amidation, gamma carboxylation of glutamic acid (E to ϒ), N-terminus conversion of Gln (Q) to a pyroglutamyl residue (Z), and hydroxylation of Pro (P) to Hyp (O) are observed in one or more peptides identified in this study. Proteolytic trimming of sequences by cleavage at the C-terminus of Asn (N) residues is established. The presence of an asparagine endopeptidase is strengthened by the identification of legumain-like sequences in the transcriptome assemblies from diverse Conus species. Such sequences may be expected to have a cleavage specificity at Asn-Xxx peptide bonds.</p>
},
	issn = {1099-1387},
	doi = {10.1002/psc.3554},
	author = {Vijayasarathy, Marimuthu and Kumar, Sanjeev and Das, Rajdeep and Balaram, Padmanabhan}
}
@article {95318,
	title = {Effect of nanoparticle exposure in a living system: probed by quantification of Fetuin-B in plasma proteome and kidney tissue imaging using MALDI imaging mass spectrometry in a rat model},
	journal = {JOURNAL OF NANOPARTICLE RESEARCH},
	volume = {23},
	year = {2021},
	month = {06/2021},
	chapter = {6},
	abstract = {<p>Nanoparticles have gained importance in various biomedical field viz cosmetics, diagnostics, therapeutics, and food additives. Due to small size, NPs penetrate tissues and cells, thereby interacting with biomolecules such as proteins and nucleic acids. This raises the major concern to investigate the effect of NPs in a living system. Previously, we reported downregulation of Fetuin-B in rat plasma, with the increasing dose level of NP-Fe-4(P2O7)(3), in rats treated with NP-Fe-4(P2O7)(3) as a food-fortificant. In the present study, we validated the dose response of FetuinB in rats exposed to NP-Fe-4(P2O7)(3), NP-ZnO, and NP-SiO2. Fetuin-B showed similar dose responses in all the groups irrespective of their physicochemical properties. Previously, we hypothesized that the immune response was triggered upon exposure to NP-Fe-4(P2O7)(3) in vivo, which led to the differential regulation of Fetuin-B. To evaluate the hypothesis, immunological response was assessed in rats by estimating the pro-inflammatory cytokines in plasma. We observed Interleukin-la and Interleukin-1 beta increased with the increasing dose levels of the three NPs. The impact of NPs on the proteomic profile in the kidney tissues of rats exposed to three NPs, showed differential spatial distribution of a-Enolase across the medulla and the cortex region with respect to the control group, suggesting an overexpression of a-Enolase. Overexpression of a-Enolase might be an indicative of cellular apoptosis in response to cytotoxicity caused due to NP perforation across the cellular membrane. In the present study, we propose that Fetuin-B might be considered a plasma biomarker to detect nanotoxicity at an early stage in a living system.</p>
},
	doi = {10.1007/s11051-021-05251-z},
	author = {Srinivasu, BY and Arumugaperumal, Arun and Mitra, Amrita and Muralidharan, Monita and Das, Rajdeep and Mandal, Amit Kumar}
}
