
@article {155676,
	title = {Ca2+ binding to Esyt modulates membrane contact site density in Drosophila photoreceptors.},
	journal = {J Cell Biol},
	volume = {224},
	year = {2025},
	month = {2025 May 05},
	abstract = {<p>Membrane contact sites (MCS) between the plasma membrane (PM) and endoplasmic reticulum (ER) regulate Ca2+ influx. However, the mechanisms by which cells modulate ER-PM MCS density are not understood, and the role of Ca2+, if any, in regulating these is unknown. We report that in Drosophila photoreceptors, MCS density is regulated by the Ca2+ channels, TRP and TRPL. Regulation of MCS density by Ca2+ is mediated by Drosophila extended synaptotagmin (dEsyt), a protein localized to ER-PM MCS and previously shown to regulate MCS density. We find that the Ca2+-binding activity of dEsyt is required for its function in vivo. dEsytCaBM, a Ca2+ non-binding mutant of dEsyt is unable to modulate MCS structure. Further, reconstitution of dEsyt null photoreceptors with dEsytCaBM is unable to rescue ER-PM MCS density and other key phenotypes. Thus, our data supports a role for Ca2+ binding to dEsyt in regulating ER-PM MCS density in photoreceptors thus tuning signal transduction during light-activated Ca2+ influx.</p>
},
	keywords = {Animals, Calcium, Calcium Signaling, Cell Membrane, Drosophila melanogaster, Drosophila Proteins, Endoplasmic Reticulum, Photoreceptor Cells, Invertebrate, Synaptotagmins, Transient Receptor Potential Channels},
	doi = {10.1083/jcb.202407190},
	author = {Nath, Vaisaly R and Krishnan, Harini and Mishra, Shirish and Raghu, Padinjat}
}
