
@article {59719,
	title = {Anillin Promotes Cell Contractility by Cyclic Resetting of RhoA Residence Kinetics.},
	journal = {Dev Cell},
	volume = {49},
	year = {2019},
	month = {2019 Jun 17},
	pages = {894-906.e12},
	abstract = {<p>RhoA stimulates cell contractility by recruiting downstream effectors to the cortical plasma membrane. We now show that direct binding by anillin is required for effective signaling: this antagonizes the otherwise labile membrane association of GTP-RhoA to promote effector recruitment. However, since its binding to RhoA blocks access by other effectors, we demonstrate that anillin must also concentrate membrane phosphoinositide-4,5-P (PIP) to promote signaling. We propose and test a sequential pathway where GTP-RhoA first binds to anillin and then is retained at the membrane by PIP after it disengages from anillin. Importantly, re-binding of membrane GTP-RhoA to anillin, regulated by the cortical density of anillin, creates cycles through this pathway. These cycles repeatedly reset the dissociation kinetics of GTP-RhoA, substantially increasing its dwell time to\&nbsp;recruit effectors. Thus, anillin regulates RhoA signaling by a paradigm of kinetic scaffolding that may apply to other signals whose efficacy depends on their cortical dwell times.</p>
},
	issn = {1878-1551},
	doi = {10.1016/j.devcel.2019.04.031},
	author = {Budnar, Srikanth and Husain, Kabir B and Gomez, Guillermo A and Naghibosadat, Maedeh and Varma, Amrita and Verma, Suzie and Hamilton, Nicholas A and Morris, Richard G and Yap, Alpha S}
}
