
@article {84575,
	title = {Asymmetric chromosome segregation and cell division in DNA damage-induced bacterial filaments.},
	journal = {Mol Biol Cell},
	volume = {31},
	year = {2020},
	month = {12/2020},
	pages = {2920-2931},
	abstract = {<p>Faithful propagation of life requires coordination of DNA replication and segregation with cell growth and division. In bacteria, this results in cell size homeostasis and periodicity in replication and division. The situation is perturbed under stress such as DNA damage, which induces filamentation as cell cycle progression is blocked to allow for repair. Mechanisms that release this morphological state for re-entry into wild type growth are unclear. Here we show that damage-induced filaments divide asymmetrically, producing short daughter cells that tend to be devoid of damage and have wild type size and growth dynamics. The Min system primarily determines division site location in the filament, with additional regulation of division completion by chromosome segregation. Collectively, we propose that coordination between chromosome (and specifically segregation and cell division may result in asymmetric division in damage-induced filaments and facilitate recovery from a stressed state.</p>
},
	issn = {1939-4586},
	doi = {10.1091/mbc.E20-08-0547},
	author = {Raghunathan, Suchitha and Chimthanawala, Afroze and Krishna, Sandeep and Vecchiarelli, Anthony G and Badrinarayanan, Anjana}
}
@article {59708,
	title = {Tracking Bacterial Chromosome Dynamics with Microfluidics-Based Live Cell Imaging.},
	journal = {Methods Mol Biol},
	volume = {2004},
	year = {2019},
	month = {2019},
	pages = {223-238},
	abstract = {<p>In bacteria, chromosomes are highly organized within the limited volume of the cell to form a nucleoid. Recent application of microscopy and chromosome conformation capture techniques have together provided a comprehensive understanding of the nature of this organization and the role of factors such as the structural maintenance of chromosomes (SMC) proteins in the establishment and maintenance of the same. In this chapter, we outline a microfluidics-based approach for live cell imaging of Escherichia coli chromosome dynamics in wild-type cells. This assay can be used to track the activity of the SMC complex, MukBEF, on DNA and assess the impact of perturbations such as DNA damage on chromosome organization and segregation.</p>
},
	issn = {1940-6029},
	doi = {10.1007/978-1-4939-9520-2_17},
	author = {Raghunathan, Suchitha and Badrinarayanan, Anjana}
}
