
@article {100626,
	title = {Cargo-specific recruitment in clathrin- and dynamin-independent endocytosis.},
	journal = {Nat Cell Biol},
	year = {2021},
	month = {2021 Oct 06},
	abstract = {<p>Spatially controlled, cargo-specific endocytosis is essential for development, tissue homeostasis and cancer invasion. Unlike cargo-specific clathrin-mediated endocytosis, the clathrin- and dynamin-independent endocytic pathway (CLIC-GEEC, CG pathway) is considered a bulk internalization route for the fluid phase, glycosylated membrane proteins and lipids. While the core molecular players of CG-endocytosis have been recently defined, evidence of cargo-specific adaptors or selective uptake of proteins for the pathway are lacking. Here we identify the actin-binding protein Swiprosin-1 (Swip1, EFHD2) as a cargo-specific adaptor for CG-endocytosis. Swip1 couples active Rab21-associated integrins with key components of the CG-endocytic machinery-Arf1, IRSp53 and actin-and is critical for integrin endocytosis. Through this function, Swip1 supports integrin-dependent cancer-cell migration and invasion, and is a negative prognostic marker in breast cancer. Our results demonstrate a previously unknown cargo selectivity for the CG pathway and a role for specific adaptors in recruitment into this endocytic route.</p>
},
	issn = {1476-4679},
	doi = {10.1038/s41556-021-00767-x},
	author = {Moreno-Layseca, Paulina and J{\"a}ntti, Niklas Z and Godbole, Rashmi and Sommer, Christian and Jacquemet, Guillaume and Al-Akhrass, Hussein and Conway, James R W and Kronqvist, Pauliina and Kallionp{\"a}{\"a}, Roosa E and Oliveira-Ferrer, Leticia and Cervero, Pasquale and Linder, Stefan and Aepfelbacher, Martin and Zauber, Henrik and Rae, James and Parton, Robert G and Disanza, Andrea and Scita, Giorgio and Mayor, Satyajit and Selbach, Matthias and Veltel, Stefan and Ivaska, Johanna}
}
@article {34255,
	title = {Small GTPases and BAR domain proteins regulate branched actin polymerisation for clathrin and dynamin-independent endocytosis.},
	journal = {Nat Commun},
	volume = {9},
	year = {2018},
	month = {2018 May 09},
	pages = {1835},
	abstract = {<p>Using real-time TIRF microscopy imaging, we identify sites of clathrin and dynamin-independent CLIC/GEEC (CG) endocytic vesicle formation. This allows spatio-temporal localisation of known molecules affecting CG endocytosis; GBF1 (a GEF for ARF1), ARF1 and CDC42\&nbsp;which appear sequentially over 60 s, preceding scission. In an RNAi screen for BAR domain proteins affecting CG endocytosis, IRSp53 and PICK1, known interactors of CDC42 and ARF1, respectively, were selected. Removal of IRSp53, a negative curvature sensing protein, abolishes CG endocytosis. Furthermore, the identification of ARP2/3 complex at CG endocytic sites, maintained in an inactive state reveals a function for PICK1, an ARP2/3 inhibitor. The spatio-temporal sequence of the arrival and disappearance of the molecules suggest a mechanism for a clathrin and dynamin-independent endocytic process. Coincident with the loss of PICK1 by GBF1-activated ARF1, CDC42 recruitment leads to the activation of IRSp53 and the ARP2/3 complex, resulting in a burst of F-actin polymerisation potentially powering scission.</p>
},
	issn = {2041-1723},
	doi = {10.1038/s41467-018-03955-w},
	author = {Sathe, Mugdha and Muthukrishnan, Gayatri and Rae, James and Disanza, Andrea and Thattai, Mukund and Scita, Giorgio and Parton, Robert G and Mayor, Satyajit}
}
