
@article {51001,
	title = {Extended Flight Bouts Require Disinhibition from GABAergic Mushroom Body Neurons.},
	journal = {Curr Biol},
	volume = {29},
	year = {2019},
	month = {2019 Jan 21},
	pages = {283-293.e5},
	abstract = {<p>Insect flight is a complex behavior that requires the integration of multiple sensory inputs with flight motor output. Although previous genetic studies identified central brain monoaminergic neurons that modulate Drosophila flight, neuro-modulatory circuits underlying sustained flight bouts remain unexplored. Certain classes of dopaminergic and octopaminergic neurons that project to the mushroom body, a higher integrating center in the insect brain, are known to modify neuronal output based on contextual cues and thereby organismal behavior. This study focuses on how monoaminergic modulation of mushroom body GABAergic output neurons (MBONs) regulates the duration of flight bouts. Octopaminergic neurons in the sub-esophageal zone stimulate central dopaminergic neurons (protocerebral anterior medial, PAM) that project to GABAergic MBONs. Either inhibition of octopaminergic and dopaminergic neurons or activation of GABAergic MBONs reduces the duration of flight bouts. Moreover, activity in the PAM neurons inhibits the GABAergic MBONs. Our data suggest that disinhibition of the identified neural circuit very likely occurs after flight initiation and is required to maintain the "flight state" when searching for distant sites, possibly related to food sources, mating partners, or a suitable egg-laying site. VIDEO ABSTRACT.</p>
},
	issn = {1879-0445},
	doi = {10.1016/j.cub.2018.11.070},
	author = {Manjila, Steffy B and Kuruvilla, Maria and Ferveur, Jean-Fran{\c c}ois and Sane, Sanjay P and Hasan, Gaiti}
}
@article {24295,
	title = {Gene regulation and species-specific evolution of free-flight odor-tracking in Drosophila.},
	journal = {Mol Biol Evol},
	volume = {35},
	year = {2018},
	month = {01/2018},
	pages = {3-15},
	abstract = {<p>The flight ability of insects has coevolved with the development of organs necessary for takeoff from the ground, and to generate and modulate lift while flying in complex environments. Flight orientation to the appropriate food source and mating partner depends on the perception and integration of multiple chemical signals. We used a wind tunnel-based assay to investigate the natural and molecular evolution of free-flight odor-tracking behavior in Drosophila. First, the comparison of female and male flies of several populations and species revealed substantial sex-related, inter- and intra-specific variations for distinct flight features. In these flies, we compared the molecular structure of desat1, a fast-evolving gene involved in multiple aspects of pheromonal communication in Drosophila. We manipulated desat1 regulation and found that both neural and non-neural tissues influence distinct flight features. Together, our data suggest that desat1 is involved in the evolution of free-flight odor-tracking behaviors in Drosophila.</p>
},
	issn = {1537-1719},
	doi = {10.1093/molbev/msx241},
	author = {Houot, Benjamin and Cazal{\'e}-Debat, Laurie and Fraichard, St{\'e}phane and Everaerts, Claude and Saxena, Nitesh and Sane, Sanjay P and Ferveur, Jean-Fran{\c c}ois}
}
