
@article {161131,
	title = {Targeted Bisulfite Sequencing and DNA Methylation Analysis in Rice Endosperm and Other Tissues.},
	journal = {Methods Mol Biol},
	volume = {2988},
	year = {2026},
	month = {2026},
	pages = {71-82},
	abstract = {<p>DNA methylation is a major epigenetic mark on chromatin. Tissue-specific DNA methylation in the regulatory regions of key genes modulates their tissue-specific expression. To study DNA methylation levels at specific sequence contexts across tissues or during various environmental conditions, whole-genome DNA methylation analysis is widely used. However, whole-genome methylation analysis is an expensive, time-consuming technique and requires specific skills. To understand the role of DNA methylation in a small number of candidate loci, targeted bisulfite (BS) PCR-sequencing analysis is an attractive option. Here, we describe a detailed method to understand DNA methylation by targeted-BS PCR analysis using rice endosperm tissues. This method can be readily extended to multiple monocots, across various tissues and various stress conditions.</p>
},
	keywords = {DNA Methylation, DNA, Plant, Endosperm, Epigenesis, Genetic, Genome, Plant, High-Throughput Nucleotide Sequencing, Oryza, Polymerase Chain Reaction, Sequence Analysis, DNA, Sulfites},
	issn = {1940-6029},
	doi = {10.1007/978-1-0716-4981-7_7},
	author = {Pal, Avik Kumar and Gandhivel, Vivek Hari-Sundar and Shivaprasad, P V}
}
@article {135859,
	title = {Novel regulators of mitochondrial genome integrity.},
	journal = {J Biosci},
	volume = {48},
	year = {2023},
	month = {2023},
	abstract = {<p>Mitochondrial genomes are unusual in that they have very low mutation rates in coding sequences when compared with animals, yeast, and bacteria. It has been proposed that the mitochondrial (mt) DNA damage is preferentially repaired by homologous recombination (HR). In this short review, we summarize and discuss the well-established and recently identified pathways that help in maintaining the integrity of plant mitochondrial genomes.</p>
},
	keywords = {Animals, DNA, Mitochondrial, Genome, Mitochondrial, Genome, Plant, Homologous Recombination, Mitochondria, Plants, Saccharomyces cerevisiae},
	issn = {0973-7138},
	author = {Nair, Ashwin and Shivaprasad, P V}
}
