
@article {91674,
	title = {Formin nanoclustering-mediated actin assembly during plant flagellin and DSF signaling.},
	journal = {Cell Rep},
	volume = {34},
	year = {2021},
	month = {2021 Mar 30},
	pages = {108884},
	abstract = {<p>Plants respond to bacterial infection acutely with actin remodeling during plant immune responses. The mechanisms by which bacterial virulence factors (VFs) modulate plant actin polymerization remain enigmatic. Here, we show that plant-type-I formin serves as the molecular sensor for actin remodeling in response to two bacterial VFs: Xanthomonas campestris pv. campestris (Xcc) diffusible signal factor (DSF), and pathogen-associated molecular pattern (PAMP) flagellin in pattern-triggered immunity (PTI). Both VFs regulate actin assembly by tuning the clustering and nucleation activity of formin on the plasma membrane (PM) at the nano-sized scale. By being integrated within the cell-wall-PM-actin cytoskeleton (CW-PM-AC) continuum, the dynamic behavior and function of formins are highly dependent on each scaffold layer{\textquoteright}s composition within the CW-PM-AC continuum during both DSF and PTI signaling. Our results reveal a central mechanism for rapid actin remodeling during plant-bacteria interactions, in which bacterial signaling molecules fine tune plant formin nanoclustering in a host mechanical-structure-dependent manner.</p>
},
	issn = {2211-1247},
	doi = {10.1016/j.celrep.2021.108884},
	author = {Ma, Zhiming and Liu, Xiaolin and Nath, Sangeeta and Sun, He and Tran, Tuan Minh and Yang, Liang and Mayor, Satyajit and Miao, Yansong}
}
@article {80781,
	title = {The bacterial quorum sensing signal DSF hijacks  sterol biosynthesis to suppress plant innate immunity.},
	journal = {Life Sci Alliance},
	volume = {3},
	year = {2020},
	month = {2020 Oct},
	abstract = {<p>Quorum sensing (QS) is a recognized phenomenon that is crucial for regulating population-related behaviors in bacteria. However, the direct specific effect of QS molecules on host biology is largely understudied. In this work, we show that the QS molecule DSF (-11-methyl-dodecenoic acid) produced by pv. can suppress pathogen-associated molecular pattern-triggered immunity (PTI) in , mediated by flagellin-induced activation of flagellin receptor FLS2. The DSF-mediated attenuation of innate immunity results from the alteration of FLS2 nanoclusters and endocytic internalization of plasma membrane FLS2. DSF altered the lipid profile of , with a particular increase in the phytosterol species, which impairs the general endocytosis pathway mediated by clathrin and FLS2 nano-clustering on the plasma membrane. The DSF effect on receptor dynamics and host immune responses could be entirely reversed by sterol removal. Together, our results highlighted the importance of sterol homeostasis to plasma membrane organization and demonstrate a novel mechanism by which pathogenic bacteria use their communicating molecule to manipulate pathogen-associated molecular pattern-triggered host immunity.</p>
},
	issn = {2575-1077},
	doi = {10.26508/lsa.202000720},
	author = {Tran, Tuan Minh and Ma, Zhiming and Triebl, Alexander and Nath, Sangeeta and Cheng, Yingying and Gong, Ben-Qiang and Han, Xiao and Wang, Junqi and Li, Jian-Feng and Wenk, Markus R and Torta, Federico and Mayor, Satyajit and Yang, Liang and Miao, Yansong}
}
